Related Experiment Video

Updated: Jan 25, 2026

Analysis of 18FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates
10:04

Analysis of 18FDG PET/CT Imaging as a Tool for Studying Mycobacterium tuberculosis Infection and Treatment in Non-human Primates

Published on: September 5, 2017

19.2K

Interferon-Gamma test for the detection of Mycobacterium tuberculosis complex infection in Macaca mulatta and other

JoAnn L Yee1, Kamm Prongay2, Brodie Miles2

  • 1California National Primate Research Center, University of California, Davis, California.

Journal of Medical Primatology
|May 7, 2019
PubMed

Insights

A new assay detects Mycobacterium tuberculosis complex infections in non-human primates by measuring interferon-gamma release. This method successfully distinguished infected from uninfected Rhesus macaques in initial evaluations.

Area of Science:

  • Veterinary Immunology
  • Infectious Diseases
  • Primate Research

Background:

  • Mycobacterium tuberculosis complex (MTBC) poses a significant threat to non-human primate (NHP) health.
  • Accurate diagnostics are crucial for managing MTBC infections in NHP populations.
  • Current diagnostic tools may have limitations in NHP models.

Purpose of the Study:

  • To develop and validate a novel diagnostic assay for MTBC infections in NHPs.
  • To utilize a cell-mediated immune response for pathogen detection.
  • To assess the assay's efficacy in a controlled experimental setting.

Main Methods:

  • An assay was formatted using commercially available reagents.
  • The assay elicits a specific immune response measured by interferon-gamma release.
  • Blood samples from Rhesus macaques experimentally infected with M tuberculosis were analyzed.

Main Results:

  • The assay demonstrated the ability to detect MTBC infections.
  • Interferon-gamma release served as a reliable indicator of infection.
  • The assay successfully distinguished between infected and uninfected Rhesus macaques.

Conclusions:

  • The developed assay is a promising tool for diagnosing MTBC infections in NHPs.
  • Interferon-gamma release assays offer a viable strategy for NHP tuberculosis surveillance.
  • Further validation in diverse NHP species and field conditions is warranted.

Keywords:
cell-mediated immune response (CMI)interferon-gamma release assay (IGRA)purified protein derivative-tuberculin (PPD)

More Related Videos

Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex09:23

Use of the Invertebrate Galleria mellonella as an Infection Model to Study the Mycobacterium tuberculosis Complex

Published on: June 30, 2019

12.5K
Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection09:11

Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection

Published on: July 16, 2019

9.6K

Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.7K
Pulmonary Tuberculosis I01:29

Pulmonary Tuberculosis I

Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
883
Pulmonary Tuberculosis II01:28

Pulmonary Tuberculosis II

Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
1.4K
Pulmonary Tuberculosis V01:28

Pulmonary Tuberculosis V

Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
563
Pulmonary Tuberculosis III01:31

Pulmonary Tuberculosis III

Tuberculosis (TB) is a contagious infection primarily affecting the lung parenchyma but which can also affect other body parts. TB can be classified based on disease development, presentation, and the affected anatomical site.
The first classification is based on the development of the disease, and it includes the following categories:
1.0K
Pulmonary Tuberculosis IV01:26

Pulmonary Tuberculosis IV

Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
499