Related Experiment Video

Updated: Dec 17, 2025

Diagnosis of Neoplasia in Barrett’s Esophagus using Vital-dye Enhanced Fluorescence Imaging
06:55

Diagnosis of Neoplasia in Barrett’s Esophagus using Vital-dye Enhanced Fluorescence Imaging

Published on: May 11, 2014

12.3K

Lamellar Inclusions within Hyperplastic Endoplasmic Reticulum in Benign Mesothelial Cells

Simon Haefliger1, Deepali Jain2, Thomas Menter1

  • 1Department of Pathology, Institute of Pathology and Medical Genetics, University Hospital of Basel, Basel, Switzerland.

Acta Cytologica
|June 30, 2020
PubMed

Insights

Cytoplasmic lamellar inclusions (LIs) in mesothelial cells are found in benign effusions, aiding differentiation from malignant cells. Electron microscopy revealed these inclusions within the rough endoplasmic reticulum.

Area of Science:

  • Cytopathology
  • Cell Biology
  • Electron Microscopy

Background:

  • Mesothelial cells in effusion cytology can exhibit cytoplasmic lamellar inclusions (LIs).
  • The nature, function, and diagnostic significance of these LIs are not well understood.

Purpose of the Study:

  • To investigate the characteristics of LIs in mesothelial cells.
  • To determine the prevalence and diagnostic utility of LIs in routine cytological practice.

Main Methods:

  • Review of 102 reactive and 90 malignant effusions (including 63 mesotheliomas and 27 carcinomas).
  • Electron microscopy analysis of LIs in one effusion specimen.

Main Results:

  • LIs were identified in 14% of benign mesothelial cells in reactive effusions and 4% in malignant effusions with carcinomatosis.
  • LIs were absent in malignant mesothelioma effusions.
  • Electron microscopy showed LIs as lamellar and tubular structures within hyperplastic rough endoplasmic reticulum (rER).

Conclusions:

  • Cytoplasmic LIs within hyperplastic rER occur in up to 14% of effusions, exclusively in benign mesothelial cells.
  • These inclusions serve as an indirect morphological marker for benign effusions.
  • LIs can assist cytologists in distinguishing between reactive and malignant mesothelial cells.
Abstract

Keywords:
Electron microscopyEndoplasmic reticulumInclusionsMesothelial cells

More Related Videos

Tubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection08:09

Tubal Cytology of the Fallopian Tube as a Promising Tool for Ovarian Cancer Early Detection

Published on: July 25, 2017

12.7K
Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue06:36

Elastic Staining on Paraffin-embedded Slides of pT3N0M0 Gastric Cancer Tissue

Published on: May 1, 2019

7.3K

Related Concept Videos

Cell Inclusions01:27

Cell Inclusions

Prokaryotic cells possess a variety of inclusions that play crucial roles in nutrient storage, metabolic processes, and environmental adaptation. These structures enable bacteria to thrive under fluctuating environmental conditions by storing essential resources and optimizing their metabolic efficiency.Carbon Storage: Poly-β-Hydroxybutyric Acid and Glycogen GranulesBacteria frequently store excess carbon in specialized granules. Poly-β-hydroxybutyric acid (PHB) granules are lipid...
616
Smooth Endoplasmic Reticulum01:21

Smooth Endoplasmic Reticulum

Smooth endoplasmic reticulum or smooth ER is a sub-organelle with specialized functions in animal cells and plant cells. It is often associated with the tubule morphology of the endoplasmic reticulum.
The ER provides optimal conditions for synthesizing steroid hormones and lipids, such as phospholipids and triglycerides. Traditionally, lipid metabolism was considered to be a smooth ER function. However, there is no direct evidence to prove that rough ER is completely excluded from lipid...
7.5K
Endoplasmic Reticulum01:39

Endoplasmic Reticulum

The Endoplasmic Reticulum (ER) in eukaryotic cells is a substantial network of interconnected membranes with diverse functions, from calcium storage to biomolecule synthesis. A primary component of the endomembrane system, the ER manufactures phospholipids critical for membrane function throughout the cell. Additionally, the two distinct regions of the ER specialize in the manufacture of specific lipids and proteins.
105.2K
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
4.5K
The Endoplasmic Reticulum01:43

The Endoplasmic Reticulum

The endoplasmic reticulum or ER makes up for more than half of the membranes in a cell and accounts for 10% of total cell volume. It is also the primary protein and lipid synthesis factory for most cell organelles, such as the Golgi apparatus, lysosomes, secretory vesicles, and the plasma membrane. Despite being the most extensive and functionally complex subcellular organelle, ER was the last to be discovered. After years of deliberation, Keith Porter and George Palade in the year 1954,...
20.6K
Classification of Epithelial Tissues: Simple Epithelium01:30

Classification of Epithelial Tissues: Simple Epithelium

Simple epithelium consists of a single layer of cells that lines body cavities and blood vessels. The shape of the cells in the epithelium reflects the function of the tissue. Cells in simple squamous epithelium appear as thin scales with flat, elliptical nuclei that mirror the form of the cell.
Because of the thinness of the cells, simple squamous epithelium is present where the rapid passage of chemical compounds is observed. For example, the endothelium that lines the capillaries and vessels...
12.5K