Biomolecular assembly on interdigitated electrode nanosensor for selective detection of insulin-like growth factor-1

Area of Science:

• Biomedical Engineering
• Electrochemistry
• Biosensing

Background:

• Insulin-like Growth Factor 1 (IGF1) plays crucial roles in cellular processes.
• Accurate quantification of IGF1 is essential for diagnosing and monitoring various diseases.
• Existing detection methods may lack the required sensitivity or specificity.

Purpose of the Study:

• To develop a sensitive electroanalytical method for quantifying IGF1.
• To utilize an InterDigitated electrode (IDE) surface for enhanced detection.
• To validate the specificity and sensitivity of the developed biosensing strategy.

Main Methods:

• Electrochemical analysis using a chemically modified InterDigitated electrode (IDE).
• Development of a sandwich assay utilizing both antibodies and aptamers for IGF1 capture.
• Characterization of the electrode surface using microscopic observations.
• Control experiments to confirm assay specificity.

Main Results:

• Achieved a limit of detection (LOD) for IGF1 as low as 10 femtomolar (fM).
• Demonstrated a 100-fold enhancement in sensitivity compared to conventional methods.
• Confirmed specific IGF1 detection, with no signal interference from IGF2 or non-complementary aptamers.
• Validated the assay's robustness through various control experiments.

Conclusions:

• The developed aptamer-antibody sandwich assay on IDE provides a highly sensitive and specific platform for IGF1 quantification.
• This electroanalytical approach offers a promising alternative for IGF1 detection in clinical and research settings.
• The strategy can complement existing imaging systems for conditions like Abdominal Aortic Aneurysm (AAA).