Related Experiment Video

Updated: Oct 10, 2025

Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens
09:15

Two Methods for Establishing Primary Human Endometrial Stromal Cells from Hysterectomy Specimens

Published on: May 23, 2014

23.1K

A study on preserving endometrial glandular architecture during preparation using BD SurePath™ liquid-based cytology

Takeshi Nishikawa1, Hisae Suzuki1, Mao Takeuchi1

  • 1Department of Pathology, Nara Medical University Hospital, Nara, Japan.

Cytopathology : Official Journal of the British Society for Clinical Cytology
|December 9, 2021
PubMed

Insights

Preserving endometrial glandular architecture in liquid-based cytology is possible. Longer fixation times (≥18 hours) with the PrepMate™ System retain the crucial three-dimensional structure of endometrial cells.

Area of Science:

  • Gynecologic Pathology
  • Cytopathology
  • Cellular Morphology

Background:

  • Disruption of the three-dimensional architecture of endometrial glands is a common issue in liquid-based cytology preparations.
  • BD SurePath™ liquid-based cytology (SP-LBC) reagents can affect the structural integrity of endometrial specimens.
  • Understanding the causes of architectural disruption is crucial for accurate cytopathological interpretation.

Purpose of the Study:

  • To determine the factors causing the disruption of endometrial glandular architecture in SP-LBC specimens.
  • To identify an optimal sample preparation method for retaining the three-dimensional structure of endometrial glands.

Main Methods:

  • Three methods of SP-LBC specimen preparation were compared: (A) BD PrepMate™ System with 1-6h fixation, (B) no PrepMate™ System with 1-6h fixation, and (C) PrepMate™ System with ≥18h fixation.
  • Evaluation of endometrial cell cluster size and number, and the count of solitary scattered cells.
  • Statistical analysis to compare the outcomes of the different preparation methods.

Main Results:

  • Method C (PrepMate™ System, ≥18h fixation) yielded significantly more large endometrial cell clusters (≥200 μm) compared to methods A and B.
  • Method B (no PrepMate™, 1-6h fixation) produced more cell clusters than method A (PrepMate™, 1-6h fixation).
  • Method A resulted in a significantly higher number of solitary scattered cells compared to methods B and C.

Conclusions:

  • Retention of endometrial glandular architecture is achievable by using the PrepMate™ System with extended fixation times (≥18 hours).
  • This optimized method allows for better preservation of the native three-dimensional structure of endometrial glands in SP-LBC.
  • The findings provide a practical approach for improving endometrial cytology specimen quality.
Abstract

Keywords:
cellular fixationendometrial cytologyendometrial glandular architectureliquid based cytologypreservative fluid

More Related Videos

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

4.2K
Isolation of Intact, Whole Mouse Mammary Glands for Analysis of Extracellular Matrix Expression and Gland Morphology12:49

Isolation of Intact, Whole Mouse Mammary Glands for Analysis of Extracellular Matrix Expression and Gland Morphology

Published on: October 30, 2017

17.1K

Related Concept Videos

Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
6.5K