Reference ranges of T lymphocyte subsets by single-platform among healthy population in southwest China
Bin Wei1, Ying Guo2, Liangjun Zhang3
1Department of Laboratory Medicine/Clinical Research Center of Laboratory Medicine, West China Hospital, Sichuan University, 37 Guo Xue Xiang, Chengdu, 610041, Sichuan Province, China.
Insights
Establishing age- and sex-specific T lymphocyte reference ranges is crucial for immunological disease assessment. This study provides new, superior reference intervals for the southwest China population, accounting for age and gender differences.
Area of Science:
- Immunology
- Clinical Chemistry
- Population Health
Background:
- Accurate T lymphocyte subset reference ranges are vital for evaluating immune status in patients with immunological diseases.
- Establishing population-specific reference intervals is essential for precise patient assessment.
Purpose of the Study:
- To establish age- and sex-related reference intervals for T lymphocyte subsets using a single-platform method in the southwest China population.
- To compare newly derived reference intervals with existing ones for improved clinical utility.
Main Methods:
- Utilized data from 53,822 individuals undergoing periodic health examinations (2018-2020).
- Employed the indirect method for reference interval determination.
- Applied Box-Cox conversion and Tukey method for data normalization and outlier removal, followed by nonparametric methods for interval estimation.
Main Results:
- Established reference ranges for T lymphocyte subsets in a healthy southwest China population using the indirect method.
- Demonstrated significant differences in T lymphocyte subset reference ranges based on age and gender.
- Confirmed a decrease in absolute counts of CD3+, CD4+, and CD8+ T cells with aging, particularly in males.
Conclusions:
- Reference intervals for T lymphocyte subsets should be differentiated by age and gender.
- The newly established reference intervals are superior to those derived from current reagent specifications.
- These findings enhance the accuracy of immune status evaluation in the target population.
Background:
Appropriate reference ranges of T lymphocyte subsets are essential for immune status evaluation of patients with immunological diseases. We aim to establish the age- and sex-related reference intervals of T lymphocyte subsets by single-platform for the southwest China population using the indirect method with the data resulting from 53,822 cases of periodic health examination individuals in the Laboratory Information System (LIS) of West China Hospital from 2018 to 2020.
Methods:
We used the Box-Cox conversion combined with the Tukey method to normalize the data and eliminate the outliers, and the nonparametric method to estimate the 95% distribution reference intervals.
Results:
We initially established the reference ranges of T lymphocyte subsets by single-platform among healthy population in southwest China by indirect method (See text for details). Using the standard normal deviate test (z-test) suggested by Harris and Boyd according to CLSI EP28-A3C, which is more scientific, we found the reference ranges of T lymphocyte subsets should be differentiated by ages and genders since the reference ranges of T lymphocyte subsets by single-platform in different ages and genders are significantly different.
Conclusions:
We further demonstrated the absolute count of CD3 + T cell, CD3 + CD4 + T cell, CD3 + CD8 + T cell decreased with aging, which is more marked in men and CD3 + CD8 + T cell count, and the obtained reference intervals were superior to the reference intervals derived from the reagent specification currently in use.
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