Related Experiment Video

Updated: Aug 28, 2025

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
11:44

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

Published on: January 24, 2016

12.0K

Bcl10 phosphorylation-dependent droplet-like condensation positively regulates DNA virus-induced innate immune

Dandan Yang1, Gaofeng Pei2,3, Shuangshuang Dong2,3

  • 1Institute for Immunology, School of Medicine, Tsinghua University, Beijing, 100084, China.

Science China. Life Sciences
|September 17, 2022
PubMed

Insights

B-cell lymphoma 10 (Bcl10) protein is crucial for innate immunity against DNA viruses. Bcl10 forms condensates, activating the STING-TBK1 pathway to boost type I interferon production and antiviral defense.

Area of Science:

  • Immunology
  • Molecular Biology
  • Virology

Background:

  • B-cell lymphoma 10 (Bcl10) is a key regulator of NF-κB signaling.
  • The role of Bcl10 in antiviral innate immunity requires further elucidation.

Purpose of the Study:

  • To investigate the function of Bcl10 in type I interferon (IFN) expression during DNA virus infection.
  • To elucidate the molecular mechanisms by which Bcl10 regulates the innate immune response to viral pathogens.

Main Methods:

  • Utilized Bcl10-deficient mice to assess susceptibility to Herpes simplex virus 1 (HSV-1) infection.
  • Investigated the recruitment and phosphorylation of Bcl10 within the STING-TBK1 signaling complex.
  • Analyzed Bcl10-dependent formation of protein condensates and signalosomes.

Main Results:

  • Bcl10-deficient mice exhibited increased susceptibility to HSV-1 infection.
  • DNA virus infection induced Bcl10 recruitment to the STING-TBK1 complex, leading to its phosphorylation by TBK1.
  • Phosphorylated Bcl10 formed droplet-like condensates, promoting TBK1 activation and subsequent IRF3 phosphorylation for type I IFN production.

Conclusions:

  • Bcl10 plays a critical role in antiviral innate immunity by regulating type I IFN expression.
  • Bcl10 induces an innate immune response through droplet-like condensation and signalosome formation downstream of the cGAS-STING pathway.

Keywords:
Bcl10DNA viruscondensationdropletphosphorylation

More Related Videos

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production08:32

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Published on: March 2, 2014

10.6K
Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells09:15

Tyramide Signal Amplification for the Immunofluorescent Staining of ZBP1-Dependent Phosphorylation of RIPK3 and MLKL After HSV-1 Infection in Human Cells

Published on: October 20, 2022

2.3K

Related Concept Videos

Immune Response Against Viral Pathogens01:29

Immune Response Against Viral Pathogens

The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
888
DNA Bacteriophages01:26

DNA Bacteriophages

Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
108
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
3.2K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
9.2K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.8K
Viral Structure00:56

Viral Structure

Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
63.2K