Related Experiment Video

Updated: Jul 20, 2025

Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus
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Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus

Published on: October 1, 2015

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Immunofluorescence Microscopy

Domenico F Galati1, David J Asai2

  • 1Biology Department, College of Science and Engineering, Western Washington University, Bellingham, Washington.

Current Protocols
|August 4, 2023
PubMed

Insights

Immunofluorescence microscopy visualizes cellular molecules using fluorescent antibodies. This guide details protocols for various cell types and advanced imaging techniques, including super-resolution microscopy.

Area of Science:

  • Cell Biology
  • Microscopy
  • Molecular Biology

Background:

  • Visualizing fluorescence-tagged molecules reveals cellular dynamics.
  • Immunofluorescence microscopy uses fluorescently labeled antibodies to detect specific molecules.
  • Effective application requires careful consideration of antigen, antibody, fixation, and imaging.

Purpose of the Study:

  • To provide detailed protocols for immunofluorescence staining and imaging.
  • To cover diverse cell types, including adherent fibroblasts and suspension Tetrahymena.
  • To enable visualization using widefield, confocal, and super-resolution microscopy.

Main Methods:

  • Protocol for immunofluorescence staining of adherent cells (fibroblasts).
  • Protocol for immunofluorescence staining of suspension cells (Tetrahymena).
  • Protocols for visualization using widefield, laser scanning confocal, and super-resolution (SRRF) microscopy.

Main Results:

  • Demonstrated protocols for successful immunofluorescence staining in fibroblasts and Tetrahymena.
  • Enabled visualization of cytoskeleton and organelles in different cell types.
  • Facilitated imaging across a range of fluorescence microscopy techniques.

Conclusions:

  • Immunofluorescence microscopy is a versatile technique for visualizing cellular structures.
  • Standardized protocols can be adapted for various cell types and advanced imaging.
  • This work provides a comprehensive resource for researchers using immunofluorescence microscopy.

Keywords:
Tetrahymenacytoskeletonfluorescenceimmunofluorescencemicrotubulestubulin antibody

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