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Proteomic dataset of non-specific interactors in Leishmania infantum

Wesley Klaysson Pereira Regatieri1, Camila Rolemberg Santana Travaglini Berti de Correia1, Felipe Roberti Teixeira1

  • 1Department of Genetics and Evolution, Federal University of São Carlos, São Paulo, Brazil.

Data in Brief
|May 29, 2026
PubMed

Insights

This study identifies non-specific protein binders in Leishmania infantum using affinity purification coupled to mass spectrometry (AP-MS/MS). The findings offer a valuable resource for interpreting proteomic interactome studies in Leishmania.

Area of Science:

  • Proteomics
  • Parasitology
  • Molecular Biology

Background:

  • Affinity purification coupled to mass spectrometry (AP-MS/MS) is crucial for mapping protein-protein interactions.
  • Interpreting AP-MS/MS data requires understanding non-specific binding events.
  • Leishmania infantum is a significant human pathogen causing visceral and cutaneous leishmaniasis.

Purpose of the Study:

  • To generate a proteomic resource of non-specific protein interactions in Leishmania infantum.
  • To identify proteins that bind non-specifically across different affinity purification systems.
  • To provide a reference dataset for improving interactome studies in Leishmania.

Main Methods:

  • Proteomic analysis of Leishmania infantum promastigotes using affinity purification coupled to mass spectrometry (AP-MS/MS).
  • Utilized HA, myc, and His affinity systems for immunoprecipitation.
  • Processed raw spectral data using MaxQuant and identified proteins via LC-MS/MS.

Main Results:

  • Identified 566 unique proteins across all conditions.
  • Discovered 60 proteins consistently detected in all three affinity systems, representing putative non-specific binders.
  • Metabolism-related proteins (30%) and ribosomal components (28%) were the most abundant functional classes among non-specific binders.

Conclusions:

  • This dataset serves as a valuable reference for background protein binding in Leishmania AP-MS/MS experiments.
  • The findings support improved interpretation of interactome studies in Leishmania.
  • The resource aids in benchmarking affinity purification strategies for Leishmania proteomic research.

Keywords:
Affinity purification–mass spectrometry (AP-MS/MS)Label-free quantification (LFQ)Leishmania infantumNon-specific protein interactionsProteomics dataset

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