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CD30-mediated signaling promotes the development of human T helper type 2-like T cells

G Del Prete1, M De Carli, M M D'Elios

  • 1Division of Clinical Immunology and Allergy, University of Florence, Italy.

The Journal of Experimental Medicine
|December 1, 1995
PubMed

Insights

CD30 signaling promotes T helper type 2 (Th2) immune responses. Activating CD30 on T cells enhances their proliferation and cytokine production, favoring Th2 development and suppressing Th1-like responses.

Area of Science:

  • Immunology
  • Cellular Biology

Background:

  • CD30 is a receptor on activated T cells, part of the tumor necrosis factor receptor superfamily.
  • Previous work indicated CD30 expression on T helper type 2 (Th2) cytokine-producing T cell clones.

Purpose of the Study:

  • To investigate the role of CD30 costimulation in T cell activation and differentiation.
  • To determine if CD30 signaling influences the development of Th1 versus Th2 immune responses.

Main Methods:

  • Using agonistic anti-CD30 monoclonal antibodies to costimulate established human Th2 and Th0 T cell clones.
  • Analyzing antigen-induced proliferation and cytokine secretion.
  • Costimulating peripheral blood mononuclear cells (PBMCs) and assessing the resulting T cell lines and clones.
  • Blocking CD30 ligand-CD30 interactions in bulk cultures.

Main Results:

  • Costimulation with anti-CD30 enhanced proliferation and cytokine secretion in established Th2 and Th0 clones.
  • Anti-CD30 treatment of PBMCs led to preferential development of Th2-like T cell lines and clones.
  • Blocking CD30-CD30L interaction shifted T cell development towards a Th1-like phenotype.

Conclusions:

  • CD30 triggering acts as a costimulatory signal for activated T cells.
  • CD30 signaling promotes the development of Th2-type immune responses.
  • CD30-CD30L interactions on antigen-presenting cells are crucial for directing T cell polarization.

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