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Monoclonal antibody 1D8 detects an activation-related molecule on human B lymphocytes and on a minor T-lymphocyte

M Nikolova1, H Taskov

  • 1National Center of Infectious and Parasitic Disease, Sofia, Bulgaria.

Hybridoma
|August 1, 1994
PubMed

Insights

A new antibody, 1D8, identifies a specific antigen on B and T lymphocytes. This antigen appears crucial in the early stages of lymphocyte activation, offering insights into immune responses.

Area of Science:

  • Immunology
  • Cell Biology
  • Monoclonal Antibody Technology

Background:

  • Human peripheral blood mononuclear cells (PBMNC) are key components of the immune system.
  • Understanding lymphocyte subpopulations and activation markers is vital for immunology research.
  • Monoclonal antibodies (MAbs) are essential tools for cell surface antigen identification.

Purpose of the Study:

  • To produce and characterize a novel monoclonal antibody, MAb 1D8.
  • To determine the reactivity of MAb 1D8 on various hematopoietic cells and malignancies.
  • To investigate the role of the 1D8 antigen in lymphocyte activation.

Main Methods:

  • Immunization of mice with nylon wool-adherent PBMNC fraction.
  • Production of murine monoclonal antibody 1D8.
  • Flow cytometry analysis of MAb 1D8 reactivity on normal cells, cell lines, and malignancies.
  • Immunoprecipitation assays to characterize the antigen.

Main Results:

  • MAb 1D8 recognizes a lymphocyte-specific surface antigen.
  • The antigen is expressed on mature B lymphocytes and a subset of T lymphocytes.
  • Expression of the 1D8 antigen changes during B and T lymphocyte activation.
  • The antigen is not a conventional glycoprotein.

Conclusions:

  • MAb 1D8 identifies a novel antigen involved in early lymphocyte activation.
  • The 1D8 antigen may serve as a marker for early activation events in lymphocytes.
  • Further characterization of the 1D8 antigen could elucidate its precise role in immune function.

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