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Updated: Jul 24, 2026
Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
Published on: September 16, 2012
[Clinical usefulness of the immunophenotypic study of circulating lymphocytes in leukemic lymphoproliferative
Insights
Immunophenotyping (IF) of B-cell chronic lymphocytic leukemia (B-CLL) reveals distinct lymphocyte changes in advanced stages. Peripheral IF in some non-Hodgkin lymphomas also showed variations from tissue profiles.
Area of Science:
- Hematology
- Immunology
- Oncology
Context:
- Peripheral blood lymphocyte immunophenotyping is crucial for diagnosing and staging lymphoid malignancies.
- B-cell chronic lymphocytic leukemia (B-CLL), prolymphocytic leukemia (PLL), and B-non-Hodgkin lymphomas (B-NHL) are distinct B-cell neoplasms.
Purpose:
- To investigate the immunophenotypic profiles of peripheral lymphocytes in patients with B-CLL, PLL, and B-NHL.
- To compare peripheral immunophenotypes with established profiles and assess variations across disease stages and lymphoma subtypes.
Summary:
- Immunophenotyping results for B-CLL and PLL were consistent with existing literature.
- In B-CLL, early stages (A and B) showed less variable immunophenotypes compared to advanced stages, which exhibited decreased CD21+ and increased CD25+ and CD38+ lymphocytes.
- Peripheral immunophenotypes aligned with tissue profiles for lymphocytic, small cleaved cell, and splenic lymphomas, but discrepancies were noted in three cases of large cell, mixed small/cleaved cell, and immunoblastic lymphomas.
Impact:
- These findings highlight stage-dependent immunophenotypic shifts in B-CLL, aiding in disease monitoring.
- Variations in peripheral immunophenotypes for certain B-NHL subtypes suggest potential complexities in diagnosis or disease classification.
- The study contributes to a better understanding of lymphocyte immunophenotyping in B-cell malignancies.
Abstract:
The immunophenotypes (IF) on peripheral lymphocytes of 24 B-CLL in different stage, 2 PLL and 14 leukemic B-non Hodgkin lymphomas were investigated. As regard to B-CLL and PLL, the results are similar to those reported so far. In stage A and B of B-CLL the IF appear less variable than in advanced stages where a decrease of CD21+ and an increase of both CD25+ and CD38+ lymphocytes were observed. In the lymphocytic, small cleaved cell lymphomas and splenic lymphomas, the peripheral IF correspond to the theoretical ones of respective lymphoma tissues. On the contrary they disagree in three cases of large cells, mixed small and cleaved cell, immunoblastic lymphomas. These features are discussed.
