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An indirect enzyme immunoassay for the mycotoxin citrinin
D Abramson1, E Usleber, E Märtlbauer
1Agriculture and Agri-Food Canada, Winnipeg Research Center, Manitoba.
Insights
A new enzyme immunoassay accurately detects the mycotoxin citrinin in buffer solutions and wheat flour. This method shows high specificity and recovery rates, offering a reliable tool for food safety analysis.
Area of Science:
- Food Science
- Analytical Chemistry
- Mycotoxicology
Background:
- Citrinin is a mycotoxin produced by several fungal species.
- Mycotoxins pose significant risks to food safety and human health.
- Accurate detection methods are crucial for monitoring citrinin contamination.
Purpose of the Study:
- To develop and validate an indirect competitive enzyme immunoassay for citrinin detection.
- To assess the assay's sensitivity, specificity, and recovery in different matrices.
Main Methods:
- Indirect competitive enzyme immunoassay (ICEIA) utilizing rabbit antisera.
- Detection of citrinin in buffer solutions and spiked wheat flour samples.
- Evaluation of cross-reactivity with related mycotoxins.
Main Results:
- The assay detected citrinin in buffer solutions at concentrations of 1–13 ng/ml.
- Minimal cross-reactivity (<0.1%) was observed with structurally similar mycotoxins.
- Recovery rates of 89–104% were achieved for citrinin spiked in wheat flour.
Conclusions:
- The developed ICEIA is a sensitive and specific method for citrinin quantification.
- The assay demonstrates excellent performance for analyzing citrinin in food matrices like wheat flour.
- This method provides a valuable tool for routine citrinin monitoring in food safety.
Abstract:
An indirect competitive enzyme immunoassay using rabbit antisera could detect citrinin in buffer solutions at 1 to 13 ng/ml (0.05 to 0.65 ng per assay). Cross-reactivity with austdiol, alternariol, ochratoxin A, and deoxynivalenol was < 0.1% relative to citrinin. Recovery of citrinin added to wheat flour at 200 to 2,000 ng/g was 89 to 104%, with a coefficient of variation of 6.9 to 13%.