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Updated: Aug 9, 2026

A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies
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A Method to Assess Fc-mediated Effector Functions Induced by Influenza Hemagglutinin Specific Antibodies

Published on: February 23, 2018

A monoclonal antibody which blocks infection with feline immunodeficiency virus identifies a possible non-CD4

M J Hosie1, B J Willett, T H Dunsford

  • 1Department of Veterinary Pathology, University of Glasgow Veterinary School, Bearsden, Scotland.

Journal of Virology
|March 1, 1993
PubMed

Insights

Monoclonal antibody vpg15 identifies a cell surface protein crucial for feline immunodeficiency virus (FIV) infection. This antibody blocks FIV entry and its target protein decreases upon infection, suggesting a novel FIV receptor.

Area of Science:

  • Immunology
  • Virology
  • Cell Biology

Background:

  • Feline immunodeficiency virus (FIV) infects domestic cats, causing an immunosuppressive disease.
  • Understanding the cellular mechanisms of FIV entry is critical for developing antiviral strategies.

Purpose of the Study:

  • To identify and characterize the cell surface molecule(s) involved in FIV entry.
  • To investigate the potential of monoclonal antibody vpg15 as a tool for studying FIV infection.

Main Methods:

  • Production and characterization of monoclonal antibody vpg15.
  • Flow cytometry to detect cell surface protein expression.
  • In vitro infection assays to assess the effect of vpg15 on FIV entry.
  • Analysis of vpg15 marker expression in FIV-infected cells.

Main Results:

  • Monoclonal antibody vpg15 specifically detects a 24-kDa cell surface protein on feline cells susceptible to FIV.
  • The vpg15 antibody effectively blocks FIV infection of permissive cells.
  • Expression of the vpg15-detected protein is significantly reduced in FIV-infected cells in vitro.

Conclusions:

  • The 24-kDa cell surface protein recognized by vpg15 is likely involved in FIV cellular entry.
  • This protein may represent a novel FIV receptor, distinct from CD4.
  • Monoclonal antibody vpg15 is a valuable reagent for investigating FIV-host interactions and potential therapeutic targets.

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