Related Experiment Video

Updated: Aug 8, 2026

Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts
07:18

Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts

Published on: June 13, 2019

Soluble intercellular adhesion molecule-1 inhibits MHC-restricted specific T cell/tumor interaction

J C Becker1, C Termeer, R E Schmidt

  • 1Department of Dermatology, University of Würzburg, Germany.

Journal of Immunology (Baltimore, Md. : 1950)
|December 15, 1993
PubMed

Insights

Soluble intercellular adhesion molecule-1 (ICAM-1) inhibits T cell-mediated melanoma killing and cytokine production. This soluble ICAM-1 blocks specific T cell interactions with melanoma, impacting immune responses.

Area of Science:

  • Immunology
  • Cell Biology
  • Oncology

Background:

  • Intercellular adhesion molecule-1 (ICAM-1)/LFA-1 pathway is crucial for immune cell adhesion and function.
  • Soluble ICAM-1 has previously shown inhibitory effects on non-MHC-restricted cytotoxicity.

Purpose of the Study:

  • To investigate the impact of soluble ICAM-1 on MHC-restricted T cell interactions with melanoma.
  • To characterize the role of soluble ICAM-1 in T cell-mediated tumor immunity.

Main Methods:

  • Established tumor-infiltrating lymphocyte (TIL) clones and autologous melanoma lines.
  • Assessed conjugate formation and MHC-restricted killing of melanoma by TIL clones.
  • Evaluated the effects of purified soluble ICAM-1 and melanoma supernatants on TIL/melanoma interactions.

Main Results:

  • Purified soluble ICAM-1 and melanoma-derived soluble ICAM-1 significantly inhibited TIL-melanoma conjugate formation.
  • Soluble ICAM-1 abrogated MHC-restricted killing of melanoma by TIL clones.
  • Soluble ICAM-1 blocked the induction of cytokines in TILs upon contact with autologous tumor cells.

Conclusions:

  • Soluble ICAM-1 effectively inhibits MHC-restricted T cell-mediated killing of melanoma.
  • Soluble ICAM-1 interferes with T cell-tumor cell interactions and subsequent immune responses.
  • Targeting soluble ICAM-1 may represent a therapeutic strategy in melanoma treatment.

More Related Videos

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells09:57

Real Time Detection of In Vitro Tumor Cell Apoptosis Induced by CD8+ T Cells to Study Immune Suppressive Functions of Tumor-infiltrating Myeloid Cells

Published on: January 29, 2019

Flow Cytometry-Based Isolation and Therapeutic Evaluation of Tumor-Infiltrating Lymphocytes in a Mouse Model of Pancreatic Cancer07:55

Flow Cytometry-Based Isolation and Therapeutic Evaluation of Tumor-Infiltrating Lymphocytes in a Mouse Model of Pancreatic Cancer

Published on: January 17, 2025

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
Cell Adhesion Molecules - Types and Functions01:20

Cell Adhesion Molecules - Types and Functions

Cell adhesion molecules (CAMs) are pivotal to multicellularity and the coordinated functioning of tissues and organ systems. They enable physical interactions between cells and provide mechanical strength to tissues. They also function as receptors for signal transmission across the plasma membrane. The CAMs are broadly classified into four families - integrins, cadherins, selectins, and immunoglobulin-like CAMs (IgCAMs).
CAM Families
The Integrin family of proteins is primarily  involved in a...
Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Tumor Immunotherapy01:27

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.