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Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
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Published on: September 16, 2012

Immunophenotyping specimens from HIV-infected persons: laboratory guidelines from the Centers for Disease Control and

J K Nicholson1

  • 1Immunology Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.

Cytometry
|March 15, 1994
PubMed

Insights

The Centers for Disease Control and Prevention (CDC) released new laboratory guidelines for counting CD4+ T-cells in individuals with human immunodeficiency virus (HIV). These guidelines aim to enhance the quality and reliability of lymphocyte immunophenotyping results.

Area of Science:

  • Clinical laboratory science
  • Immunology
  • Virology

Background:

  • Accurate enumeration of CD4+ T-cells is crucial for managing human immunodeficiency virus (HIV) infection.
  • Existing laboratory practices for lymphocyte immunophenotyping can vary, impacting result reliability.
  • The Centers for Disease Control and Prevention (CDC) has historically provided guidance for laboratory testing.

Purpose of the Study:

  • To present updated laboratory guidelines for CD4+ T-cell enumeration in HIV-infected individuals.
  • To address new data clarifying variables within the immunophenotyping process.
  • To improve the quality and consistency of lymphocyte immunophenotyping.

Main Methods:

  • Review and synthesis of new data related to immunophenotyping.
  • Inclusion of updated information on hematology and immunophenotyping procedures.
  • Emphasis on critical quality control parameters for reliable results.

Main Results:

  • Publication of comprehensive laboratory guidelines by the CDC.
  • Clarification of key variables affecting immunophenotyping accuracy.
  • Identification of essential quality control measures for consistent laboratory performance.

Conclusions:

  • Adherence to these guidelines will improve the quality of CD4+ T-cell enumeration.
  • Enhanced quality control ensures more reliable diagnostic and monitoring information for HIV patients.
  • These updated guidelines support standardized and high-quality laboratory practices in HIV care.

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