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Quality assessment for immunophenotyping of leukaemias and lymphomas: the Dutch experience. The SIHON Study Group
1Department of Haematology, Rotterdam, The Netherlands.
Insights
Immunophenotyping for leukaemia and lymphoma diagnosis showed high diagnostic agreement (>90%) despite varied cell percentage results between labs. Further studies aim to assess if standardizing flow cytometry methods improves result comparability.
Area of Science:
- Hematology
- Immunology
- Clinical Pathology
Background:
- Immunophenotyping is crucial for diagnosing leukaemias and lymphomas.
- Inter-laboratory variability in flow cytometry results can impact diagnostic accuracy.
Purpose of the Study:
- To assess diagnostic agreement in immunophenotyping for leukaemias and lymphomas across different laboratories.
- To investigate the impact of standardized antibody panels and interpretation guidelines on diagnostic concordance.
Main Methods:
- Regular distribution of biological samples to participating laboratories.
- Analysis of immunophenotyping data using standardized antibody panels and interpretation guidelines.
- Comparison of diagnostic outcomes and percentage of positive cells between laboratories.
Main Results:
- High diagnostic agreement (>90%) was observed among laboratories for leukaemias and lymphomas.
- Significant variability was noted in the percentage of positive cells reported by different laboratories.
- Standardized antibody panels and interpretation guidelines were implemented, but individual laboratory methods varied.
Conclusions:
- Current immunophenotyping practices achieve high diagnostic accuracy for leukaemias and lymphomas despite inter-laboratory variations in cell percentage reporting.
- Further standardization of flow cytometry methods is being explored to enhance the comparability of quantitative results.
Abstract:
Sending material at regular intervals to laboratories that perform immunophenotyping of leukaemias and lymphomas showed a large variety in the percentage of positive cells between the different laboratories but an agreement in diagnosis of more than 90%. Each laboratory used his own methods but antibody panels and guide-lines for interpretation were standardised. Studies are in progress to answer the question if standardisation in flow cytometry will result in more comparable results.
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