[Effect of human colostrum and its fraction on functional activity of B-lymphocytes]
Insights
Human colostrum enhances mouse splenocyte proliferation and immunoglobulin (Ig) synthesis, particularly IgG2a. Bovine colostrum, however, suppresses these responses, linked to bovine globulins.
Area of Science:
- Immunology
- Biochemistry
Context:
- Investigating the differential immunomodulatory effects of human and bovine colostrum on immune cells.
- Examining the impact of lipopolysaccharide (LPS) stimulation on mouse splenocytes in the presence of colostrum.
Purpose:
- To determine the influence of human and bovine colostrum on splenocyte proliferation and immunoglobulin (Ig) synthesis.
- To identify the specific components within colostrum responsible for these immunomodulatory effects.
Summary:
- Human colostrum, even at suboptimal lipopolysaccharide (LPS) doses, stimulated mouse splenocyte proliferation and Ig synthesis in a dose-dependent manner, notably increasing IgG2a-secreting cells.
- Bovine colostrum exhibited a suppressive effect on splenocyte proliferation and Ig synthesis induced by LPS. This suppression was attributed to bovine colostral globulins, not casein.
- A B-cell promoting fraction with a molecular weight of 70-75 kD was isolated from human colostrum using HPLC gel-filtration.
Impact:
- Highlights the distinct immunomodulatory properties of human versus bovine colostrum.
- Identifies potential bioactive components in human colostrum that promote B-cell activity and Ig production.
- Provides insights into the mechanisms underlying colostrum's effects on the immune system, relevant for nutritional immunology and therapeutic applications.
Abstract:
The human colostrum stimulated by suboptimal doses (50 ng/ml) of lipopolysaccharide (LPS) has induced proliferation of mouse splenocytes. Addition of the human colostrum has elicited a rise in splenocyte culture supernatants. Effect of human colostrum on Ig synthesis was dose-dependent and was expressed at 0.5% concentrations in the medium. In contrast to the human colostrum, the bovine colostrum exerted a suppressive effect on proliferation of mouse splenocytes and Ig synthesis induced by suboptimal doses of LPS. Bovine casein did not significantly affect the Ig synthesis by mouse splenocytes. Suppression of the Ig synthesis was associated with bovine colostral globulins. Using the ELISPOT method we have found that a rise in the Ig level in the culture after treatment with the human colostrum was due to an increase of the number of IgG2a-secreting cells. B-cell promoting fraction was found after HPLC gel-filtration. The molecular weight of this fraction was 70-75 kD.
