Constitutive production of interleukin-8 (IL-8) by normal and malignant human B-cells and other cell types

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M D Srivastava1, R Srivastava, B I Srivastava

  • 1Roswell Park Cancer Institute, Department of Laboratory Medicine, Buffalo, NY 14263.

Leukemia Research
|December 1, 1993
PubMed

Insights

This study found that interleukin-8 (IL-8) is produced by many more human cell types than previously thought. This discovery suggests novel immune functions for cells like B-cells.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • Interleukin-8 (IL-8) is a key cytokine involved in immune responses.
  • Previous research suggested limited cell types produce IL-8.

Purpose of the Study:

  • To investigate the range of human cell lines and primary cells capable of producing IL-8.
  • To explore the implications of broader IL-8 production for immune functions.

Main Methods:

  • Enzyme-linked immunosorbent assay (ELISA) was used to detect IL-8 in culture supernatants.
  • Indirect immunofluorescence and DNA probing with IL-8 cDNA were employed.
  • IL-8 mRNA expression was analyzed using specific probes.

Main Results:

  • Constitutive IL-8 production was detected in a wide array of cell lines, including B-cell lines, T-cell lines, myeloid, monocytoid, ovarian carcinoma, and normal fibroblasts.
  • Normal peripheral blood B-lymphocytes also produced IL-8.
  • Analysis of DNA and mRNA confirmed IL-8 gene presence and expression in producing cells.

Conclusions:

  • IL-8 is produced by a more diverse set of human cells than previously recognized.
  • This broader production suggests novel IL-8-mediated immune functions, particularly involving B-cells.

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