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A translocation (8;14) in a cutaneous large B-cell lymphoma
A M Busschots1, M L Geerts, C Mecucci
1Centre for Human Genetics, University of Leuven, Belgium.
Insights
This study details a rare case of cutaneous large-cell lymphoma in a 62-year-old man, identifying specific chromosomal abnormalities and Epstein-Barr virus association. Findings contribute to understanding B-cell non-Hodgkin
Area of Science:
- Oncology
- Genetics
- Immunology
Background:
- Cutaneous lymphomas represent a diverse group of non-Hodgkin lymphomas.
- Understanding the specific genetic and viral factors is crucial for accurate diagnosis and treatment.
Observation:
- A 62-year-old male presented with facial lesions and cervical lymphadenopathy, diagnosed as cutaneous large-cell lymphoma.
- Cytogenetic analysis revealed a complex karyotype: 47,XY,inv dup(1)(q11-->q32),+3,t(8;14)(q24;q32).
- Immunogenotyping confirmed immunoglobulin gene rearrangement, and serology was positive for Epstein-Barr virus (EBV) antigens.
Findings:
- The t(8;14)(q24;q32) translocation, common in Burkitt's lymphoma, was identified.
- Additional chromosomal aberrations included duplication of chromosome 1 long arm material and trisomy 3.
- While EBV antibodies were present, EBV DNA integration into tumor tissue was not detected via PCR.
Implications:
- The identified genetic profile provides insights into the biology of this specific cutaneous B-cell non-Hodgkin lymphoma.
- Correlation of cytogenetic findings with EBV status may aid in classifying and potentially managing similar cases.
- This case highlights the importance of comprehensive molecular analysis in non-Hodgkin lymphoma diagnosis.
Abstract:
A 62-year-old man with a cutaneous large-cell (cleaved, noncleaved) lymphoma had multiple facial lesions and two enlarged cervical lymph nodes. Cytogenetic analysis performed on the involved skin and lymph node revealed the following karyotype: 47,XY,inv dup(1)(q11-->q32),+3,t(8;14)(q24;q32). A t(8;14)(q24;q32) translocation is the chromosome anomaly in 75% of Burkitt's lymphomas. Other types of non-Hodgkin's lymphomas, however, may show the same translocation, especially large-cell lymphomas. Duplication of material belonging to the long arm of chromosome 1 is the most frequent additional aberration to Burkitt's translocations in Burkitt's lymphoma/leukemia. Trisomy 3 may be seen in both B- and T-cell malignancies, as well as in angioimmunoblastic lymphadenopathy. Immunogenotyping of malignant cells from a lymph node showed rearrangement of the immunoglobulin heavy and lambda light chain genes. Epstein-Barr virus (EBV) serology was positive for EBV nuclear antigen and EBV capsular antigen. No integration of EBV DNA in tumor tissue, however, could be detected by polymerase chain reaction. These results may be useful in defining the biologic characteristics of cutaneous B-cell non-Hodgkin's lymphomas.
