Related Experiment Video

Updated: Aug 8, 2026

Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli
14:05

Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli

Published on: November 4, 2022

Neutrophil cytotoxicity induced by immune complexes prepared with cationized antibodies

J R Geffner1, A S Trevani, E Malchiodi

  • 1Instituto de Investigaciones Hematológicas (IIHEMA), Academia Nacional de Medicina, Buenos Aires, Argentina.

Scandinavian Journal of Immunology
|February 1, 1993
PubMed

Insights

Cationized immune complexes (IC) strongly induce neutrophil-mediated cytotoxicity via oxygen-dependent pathways. These findings reveal distinct mechanisms for cationized versus control IC interactions with neutrophils.

Area of Science:

  • Immunology
  • Cellular Biology

Background:

  • Soluble immune complexes (IC) play a crucial role in immune responses.
  • Neutrophils are key effector cells in innate immunity, capable of mediating cytotoxicity.

Purpose of the Study:

  • To investigate the ability of cationized immune complexes (IC) to induce neutrophil-mediated cytotoxicity.
  • To elucidate the mechanisms underlying the cytotoxic responses induced by cationized IC.

Main Methods:

  • Preparation of cationized and control IC using antibodies.
  • Assessment of neutrophil-mediated cytotoxicity.
  • Enzyme inhibition assays (catalase, haemenzyme inhibitors).
  • Pharmacological modulation using cytochalasin B and colchicine.
  • Blocking studies with monoclonal antibodies against Fc gamma receptors (Fc gamma RII, Fc gamma RIII).
  • Enzymatic treatment of neutrophils (chemotrypsin, pronase).

Main Results:

  • Cationized IC induced significantly higher levels of neutrophil cytotoxicity compared to control IC.
  • Cytotoxicity was oxygen-dependent but myeloperoxidase-independent.
  • Cationized IC-induced cytotoxicity was inhibited by cytochalasin B and enhanced by colchicine, unlike control IC.
  • Monoclonal antibodies to Fc gamma RII completely abrogated cationized IC-induced cytotoxicity.
  • Neutrophil treatment with chemotrypsin or pronase enhanced cationized IC-induced cytotoxicity.
  • Non-specific absorptive mechanisms were involved in cationized IC binding to neutrophils.

Conclusions:

  • Cationized IC potently activate neutrophils to induce cytotoxicity through specific Fc gamma RII-dependent and oxygen-dependent pathways.
  • Distinct mechanisms govern the interaction of cationized and control IC with neutrophils.
  • These findings contribute to understanding immune complex-mediated inflammatory responses.

More Related Videos

Neutrophil Lifespan Extension with CLON-G and an In Vitro Spontaneous Death Assay05:52

Neutrophil Lifespan Extension with CLON-G and an In Vitro Spontaneous Death Assay

Published on: May 12, 2023

Real-Time, High-Throughput Microscopic Quantification of Human Neutrophil Extracellular Trap Release and Assessing the Pharmacology of Antagonists11:32

Real-Time, High-Throughput Microscopic Quantification of Human Neutrophil Extracellular Trap Release and Assessing the Pharmacology of Antagonists

Published on: October 18, 2024

Related Concept Videos

Cells of the Innate Immune Response01:28

Cells of the Innate Immune Response

The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Antibody Actions01:26

Antibody Actions

Antibodies, or immunoglobulins, are critical players in the immune system's arsenal against invading pathogens. Produced by B cells and plasma cells, their primary role is to detect and bind to specific antigens, molecules found on the surface of pathogens like bacteria or viruses. Beyond antigen recognition, antibodies perform several vital functions that contribute to immune defense.
Neutralization
Antibodies can bind to pathogens, preventing them from infecting host cells. This process...
Hypersensitivity Reactions: Cytolytic Reactions01:01

Hypersensitivity Reactions: Cytolytic Reactions

Type II hypersensitivity involves IgG and IgM antibodies targeting cell surface antigens, leading to cell destruction. This can occur through complement activation, antibody-dependent cell-mediated cytotoxicity (ADCC), or acting as opsonins for phagocytosis. When excessive, these reactions cause significant tissue damage.Drug-induced hemolytic anemia is a common example, where drugs like penicillin or cephalosporins bind to red blood cells, forming drug-protein complexes. These complexes...
Hypersensitivity Reactions: Immune-Complex Reactions01:19

Hypersensitivity Reactions: Immune-Complex Reactions

Type III hypersensitivity reactions occur when antigen–antibody complexes form and activate the complement system. Normally, these complexes help the clearance of antigens by phagocytes and red blood cells. However, when large numbers of immune complexes are present, they can deposit in tissues—particularly in the walls of blood vessels—leading to inflammation and tissue injury. These deposits trigger complement activation and neutrophil recruitment, resulting in serum sickness, a systemic...