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Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay
14:45

Enumeration of Major Peripheral Blood Leukocyte Populations for Multicenter Clinical Trials Using a Whole Blood Phenotyping Assay

Published on: September 16, 2012

Blood cell markers

C B Grindem1

  • 1Department of Microbiology, Pathology, and Parasitology, College of Veterinary Medicine, North Carolina State University, Raleigh, USA.

The Veterinary Clinics of North America. Small Animal Practice
|September 1, 1996
PubMed

Insights

Flow cytometry and monoclonal antibodies aid in diagnosing canine and feline lymphomas and immunodeficiencies. These immunophenotyping techniques enhance blood cell and cancer characterization in veterinary medicine.

Area of Science:

  • Veterinary Hematology
  • Immunology
  • Oncology

Background:

  • Morphologic characterization of blood cells and neoplasms is crucial.
  • Cytochemistry and immunocytochemistry are established diagnostic tools.
  • Flow cytometry and monoclonal antibodies offer advanced capabilities.

Purpose of the Study:

  • To highlight the clinical applications of flow cytometry and leukocyte monoclonal antibodies in veterinary medicine.
  • To discuss the classification and significance of immunophenotyping for canine and feline lymphomas.
  • To review immunophenotyping in feline immunodeficiencies, including feline immunodeficiency virus and feline leukemia virus.

Main Methods:

  • Utilizing flow cytometry for cellular analysis.
  • Employing leukocyte monoclonal antibodies for immunophenotyping.
  • Applying cytochemistry as a complementary diagnostic method.

Main Results:

  • Demonstrated the utility of flow cytometry and monoclonal antibodies in classifying canine and feline lymphomas.
  • Showcased the role of immunophenotyping in diagnosing feline immunodeficiency virus and feline leukemia virus.
  • Highlighted the importance of these techniques in veterinary hematologic diagnostics.

Conclusions:

  • Flow cytometry and monoclonal antibodies are essential for accurate diagnosis and classification of hematologic disorders in companion animals.
  • Immunophenotyping provides critical insights into lymphomas and viral immunodeficiencies in cats and dogs.
  • These advanced technologies significantly improve diagnostic capabilities in veterinary medicine.

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