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Published on: February 8, 2016
Flow cytometric immunophenotyping of non-Hodgkin's lymphomas and related disorders
1Department of Clinical Pathology, Cleveland Clinic Foundation, OH 44195, USA.
Insights
Non-Hodgkin's lymphomas (NHL) are immune system cancers. Multicolor flow cytometry aids in diagnosing specific NHLs and related disorders, though its role in lymph node biopsy evaluation lacks consensus.
Area of Science:
- Hematology
- Immunology
- Oncology
Background:
- Non-Hodgkin's lymphomas (NHL) are recognized as neoplasms of the immune system.
- Extensive research has elucidated the immunophenotypic and functional aspects of neoplastic lymphocytes.
- A lack of consensus exists regarding the precise utility of immunotypic and genotypic ancillary procedures in lymph node biopsy analysis.
Purpose of the Study:
- To review the existing literature on the role of ancillary procedures in NHL diagnosis.
- To provide an overview of multicolor flow cytometry's application in diagnosing NHL and related conditions.
Main Methods:
- Literature review of selected studies.
- Overview of multicolor flow cytometry techniques.
Main Results:
- Significant advancements in understanding neoplastic lymphocytes have been made over two decades.
- Multicolor flow cytometry is a valuable tool for diagnosing specific NHL subtypes and related disorders.
Conclusions:
- Despite progress, the exact role of immunotypic and genotypic tests in lymph node biopsies remains debated.
- Multicolor flow cytometry offers a crucial method for accurate NHL diagnosis and classification.
Abstract:
More than two decades have past since the recognition of non-Hodgkin's lymphomas (NHLs) as neoplasms of the immune system. During that time, a vast literature and knowledge base regarding the immunophenotypic and functional characteristics of neoplastic lymphocytes has been developed. Despite the accumulated wealth of knowledge, there remains no consensus as to the exact role of immunotypic and genotypic ancillary procedures in the evaluation of a lymph node biopsy. We review selected literature in this regard and provide an overview of the role of multicolor flow cytometry in establishing the diagnosis of specific NHL and related disorders.
