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1997 revised guidelines for performing CD4+ T-cell determinations in persons infected with human immunodeficiency
Insights
Updated CDC guidelines provide recommendations for lymphocyte immunophenotyping assays in laboratories serving people with human immunodeficiency virus (HIV). These guidelines reflect current technology and cover all aspects of laboratory testing for HIV monitoring.
Area of Science:
- Clinical laboratory science
- Immunology
- Virology
Background:
- Lymphocyte immunophenotyping is crucial for monitoring human immunodeficiency virus (HIV) infection.
- Rapid technological advancements necessitate updated laboratory guidelines.
- Previous recommendations require revision to align with current practices.
Framework:
- Revised guidelines from the Centers for Disease Control and Prevention (CDC).
- Focus on laboratories performing lymphocyte immunophenotyping assays.
- Target population: individuals with HIV.
Implementation:
- Recommendations cover laboratory safety and specimen handling (collection, transport, integrity, processing).
- Guidance on flow cytometer quality control, sample, and data analysis.
- Includes protocols for data storage, reporting, and overall quality assurance.
Implications:
- Ensures accurate and reliable immunophenotyping results for HIV patient management.
- Promotes standardization of laboratory practices in a rapidly evolving field.
- Aims to improve the quality of care for individuals living with HIV through enhanced laboratory services.
Abstract:
These revised guidelines were developed by CDC for laboratories performing lymphocyte immunophenotyping assays in human immunodeficiency virus-infected persons. This report updates previous recommendations (MMWR 43[No. RR-3]) and reflects current technology in a field that is rapidly changing. The recommendations address laboratory safety, specimen collection, specimen transport, maintenance of specimen integrity, specimen processing, flow cytometer quality control, sample analyses, data analysis, data storage, data reporting, and quality assurance.