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Updated: Aug 8, 2026
Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
Published on: April 8, 2016
Immunoperoxidase techniques in the evaluation of cutaneous lymphocytic infiltrates
1Department of Pathology, Stanford University Medical Center, CA 94305, USA.
Insights
Immunophenotyping enhances the diagnosis of skin lymphoid disorders. This technique aids in differentiating lymphomas from similar conditions in dermatopathology.
Area of Science:
- Dermatopathology
- Immunopathology
- Oncology
Background:
- Immunoperoxidase technology and antibody probes have significantly advanced dermatopathology over 15 years.
- Microscopic study of cutaneous lymphoid infiltrates has been particularly transformed.
- Accurate diagnosis of skin lymphomas remains a challenge.
Purpose of the Study:
- To review the application of immunophenotyping in diagnosing cutaneous lymphoproliferative disorders.
- To propose a strategy for using immunophenotyping in clinical practice.
- To highlight the role of immunophenotyping in differential diagnosis.
Main Methods:
- Review of immunophenotyping techniques in dermatopathology.
- Analysis of immunopathologic findings in cutaneous lymphoid infiltrates.
- Application of immunophenotyping for lymphoma diagnosis.
Main Results:
- Immunophenotyping provides crucial data for diagnosing cutaneous lymphoproliferative disorders.
- The technique is effective in distinguishing lymphomas from their mimics.
- A strategic approach to immunophenotyping aids in clinical decision-making.
Conclusions:
- Immunophenotyping is indispensable in modern dermatopathology for diagnosing skin lymphomas.
- It resolves diagnostic challenges in differentiating lymphoma from non-lymphoma mimics.
- The review provides a framework for utilizing immunophenotyping effectively.
Abstract:
The widespread availability of immunoperoxidase technology and antibody probes over the past 15 years has changed the discipline of dermatopathology. In no aspect is this more evident than in the microscopic study of cutaneous lymphoid infiltrates. In the following review, the discussion focuses on some of the ways in which immunophenotyping is used to enhance the diagnosis of cutaneous lymphoproliferative disorders. A strategy for the use of immunophenotyping in some common clinical situations is forwarded. The immunopathologic findings can be helpful in resolving problems in the differential diagnosis of lymphoma and mimics of lymphoma.
