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Glomerular proliferative activity and T lymphocyte infiltration in acute serum sickness
G P Borges1, P Moreno, B Rodríguez Iturbe
1Centro de Cirugía Experimental, Hospital Universitario de Maracaibo, Universidad del Zulia, Venezuela.
Insights
In acute serum sickness (AcSS), increased lymphocyte infiltration correlates with glomerular proliferation, especially in rabbits with proteinuria. This highlights a key relationship in disease development.
Area of Science:
- Nephrology
- Immunology
- Pathology
Background:
- Hypercellularity is a key feature of acute serum sickness (AcSS).
- Understanding glomerular changes is crucial for AcSS pathogenesis.
Purpose of the Study:
- To quantify glomerular proliferation in AcSS using anti-proliferating cell nuclear antigen (PCNA).
- To investigate the relationship between glomerular proliferation and lymphocyte infiltration in AcSS.
Main Methods:
- Acquired data from 31 New Zealand White rabbits with induced AcSS, categorized by proteinuria and sacrifice time.
- Quantified intraglomerular proliferation using PCNA staining and lymphocyte infiltration with M108 Mab.
- Correlated proliferation and infiltration with IgG and C3 deposition and proteinuria severity.
Main Results:
- Intraglomerular proliferation significantly increased in rabbits with proteinuria (Group A).
- Lymphocyte infiltration increased over time and was more pronounced in rabbits with proteinuria.
- Lymphocyte infiltration showed a strong positive correlation with proliferative activity (r = 0.67, P < 0.0001).
Conclusions:
- Demonstrated a chronological association between lymphocyte infiltration and glomerular proliferative activity in AcSS.
- Glomerular proliferation and proteinuria severity correlated with IgG and C3 deposition.
Abstract:
Because hypercellularity is an important feature in acute serum sickness (AcSS), we quantified glomerular proliferation with immunoperoxidase staining using anti-proliferating cell nuclear antigen (PCNA Mab) and studied its relationship with lymphocyte infiltration (M108 Mab). AcSS was induced in 31 New Zealand White rabbits; group A (n = 14), with proteinuria, sacrificed 6-8 days after immunization; group B (n = 10), without proteinuria, sacrificed 6-7 days after immunization; group C (n = 7), sacrificed prior to development of AcSS. Four normal rabbits were included as controls. Intraglomerular proliferation (PCNA-positive cells/glomerular cross section) was increased in group A (12.2 +/- SEM, 1.84) but not in groups B (0.93 +/- 0.17) and C (0.37 +/- 0.05), which were similar to controls (0.66 +/- 0.06). Lymphocyte infiltration (lymphs/glomerular cross section) increased with time and was more prominent in rabbits with proteinuria (1.9 +/- 0.21, P < 0.001 vs controls). Lymphocyte infiltration was correlated with proliferative activity (Spearman correlation, r = 0.67, P < 0.0001). There was correlation between the severity of glomerular deposition of IgG and C3 and glomerular proliferation and proteinuria. These studies demonstrate a chronological association between lymphocyte infiltration and proliferative activity in AcSS.