Related Experiment Video

Updated: Aug 8, 2026

Artificial Antigen Presenting Cell (aAPC) Mediated Activation and Expansion of Natural Killer T Cells
13:18

Artificial Antigen Presenting Cell (aAPC) Mediated Activation and Expansion of Natural Killer T Cells

Published on: December 29, 2012

Domon L, an immunopotentiating agent, acts as an NF-kappaB activator

S Tanaka1, H Sakurai, M Sugiura

  • 1Lead Generation Research Laboratory, Tanabe Seiyaku Co., Ltd., Osaka, Japan.

The Journal of Veterinary Medical Science
|July 1, 1997
PubMed

Insights

Domon L, derived from Achromobacter stenohalis, boosts the immune system by enhancing nitric oxide (NO) production and activating NF-kappaB. This immunopotentiating effect suggests its potential as an anti-infectious agent in veterinary medicine.

Area of Science:

  • Immunology
  • Microbiology
  • Molecular Biology

Background:

  • Domon L is a heat-treated component of Achromobacter stenohalis.
  • It is utilized for treating infectious diseases in animals.

Purpose of the Study:

  • To investigate the immunopotentiating potential of Domon L.
  • To elucidate the molecular mechanisms underlying Domon L's effects.

Main Methods:

  • In vitro studies using murine macrophage RAW264.7 cells.
  • Assessing nitric oxide (NO) formation and interferon-gamma (IFN-γ) synergy.
  • Investigating Nuclear Factor-kappaB (NF-κB) activation via nuclear translocation and reporter gene assays.
  • In vivo studies in rats measuring serum IL-6 and mucoprotein levels.
  • Ex vivo analysis of spleen cell NO production.

Main Results:

  • Domon L enhanced NO formation in RAW264.7 cells, particularly with IFN-γ.
  • Domon L induced NF-κB nuclear translocation and activated NF-κB-dependent gene expression.
  • In vivo, Domon L increased serum IL-6 and mucoprotein levels.
  • Spleen cells from Domon L-treated rats showed significantly higher NO production upon stimulation.

Conclusions:

  • Domon L exhibits immunopotentiating properties.
  • The mechanism involves the activation of the NF-κB signaling pathway.
  • Domon L demonstrates potential as an anti-infectious agent through immune modulation.

More Related Videos

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells08:42

Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells

Published on: January 15, 2018

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo08:19

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo

Published on: July 20, 2019

Related Concept Videos

NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-kB-dependent Signaling Pathway02:26

NF-kB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...