Related Experiment Video

Updated: Aug 8, 2026

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
09:25

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

Incorporation of cells into an ELISA system enhances antigen-driven lymphokine detection

J T Beech1, T Bainbridge, S J Thompson

  • 1Department of Pathology and Microbiology, Medical School, University of Bristol, UK.

Journal of Immunological Methods
|July 14, 1997
PubMed

Insights

Developing a sensitive cell-based ELISA (celELISA) improves detection of T-helper 1 (Th1) and T-helper 2 (Th2) cytokines in T-cell responses. This technique enhances measurement of key cytokines like IL-2, IL-4, and IFN-gamma.

Area of Science:

  • Immunology
  • Cellular Biology
  • Biotechnology

Background:

  • Measuring Th1/Th2 cytokine levels in T-cell responses is challenging.
  • Existing methods lack sufficient sensitivity or quantification capabilities.

Purpose of the Study:

  • To develop a highly sensitive cell-based ELISA (celELISA) for detecting murine Th1 and Th2 cytokines.
  • To improve the measurement of cytokines during in vitro antigen-driven T-cell responses.

Main Methods:

  • Development of a novel cell-based ELISA (celELISA) technique.
  • Combining principles of sandwich ELISA and ELISPOT assays for enhanced sensitivity.
  • Application in detecting cytokines from murine T-cell responses.

Main Results:

  • The celELISA demonstrated high sensitivity for detecting Th1 and Th2 cytokines.
  • Significantly improved detection of Interleukin-2 (IL-2) and Interleukin-4 (IL-4).
  • Enhanced, though lesser, detection of Interferon-gamma (IFN-gamma).

Conclusions:

  • The celELISA is a valuable tool for sensitive cytokine detection in T-cell immunology.
  • This method overcomes limitations of conventional assays for Th1/Th2 cytokine measurement.
  • Facilitates better understanding of T-cell mediated immune responses.

More Related Videos

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay06:15

Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

Published on: September 7, 2018

In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D08:15

In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D

Published on: December 9, 2022

Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...