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The receptor function of CD2 in human CD2 transgenic mice is based on highly conserved associations with signal

M K Wild1, A M Verhagen, S C Meuer

  • 1Institute for Immunology, Ruprecht-Karls-Universität, Heidelberg, Germany.

Cellular Immunology
|October 28, 1997
PubMed

Insights

This study shows that human CD2 (huCD2) engages similar signaling proteins in both human and transgenic mouse T cells. This suggests conserved molecular mechanisms for T cell activation via CD2 in different species.

Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Signaling

Background:

  • T cell activation via CD2 typically requires specific monoclonal antibodies (mAbs) targeting distinct epitopes.
  • Understanding the molecular interactions of CD2 is crucial for T cell activation research.

Purpose of the Study:

  • To investigate the proliferative response of human and transgenic mouse T cells to novel CD2 mAbs.
  • To map the epitopes of mitogenic CD2 mAbs using CD2:CD58 chimeric proteins.
  • To analyze the molecular interactions of human CD2 (huCD2) with signaling proteins in transgenic murine T cells.

Main Methods:

  • Examined T cell proliferation using novel CD2 monoclonal antibodies (AICD2.M1 and AICD2.M2).
  • Epitope mapping of CD2 mAbs utilizing CD2:CD58 chimeric proteins.
  • Analyzed molecular associations between huCD2 and signaling proteins in transgenic murine T cells via co-immunoprecipitation.

Main Results:

  • Identified novel mitogenic CD2 mAbs and partially mapped their epitopes.
  • Demonstrated that transgenic huCD2 interacts with murine tyrosine kinases (p56lck, p59fyn) and TCR/CD3 signaling complex components (CD3-epsilon, zeta chains).
  • Observed co-immunoprecipitation of tyrosine phosphatase activity with huCD2, similar to human T cells.

Conclusions:

  • Human CD2 couples to conserved signal transduction pathways in both human and transgenic mouse T cells.
  • The molecular interactions of huCD2 in transgenic mice mirror those in human T cells, validating the model system.
  • These findings provide insights into the molecular basis of T cell activation mediated by CD2.

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