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A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
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A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4

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The chemokine receptor CCR8 is preferentially expressed in Th2 but not Th1 cells

A Zingoni1, H Soto, J A Hedrick

  • 1Laboratory of Pathophysiology, Regina Elena Cancer Institute, Rome, Italy.

Journal of Immunology (Baltimore, Md. : 1950)
|July 22, 1998
PubMed

Insights

Researchers cloned and characterized mouse CCR8, finding its expression linked to Th2 immune responses. This suggests CCR8 may be a therapeutic target for allergic diseases.

Area of Science:

  • Immunology
  • Molecular Biology

Background:

  • Chemokine receptors play crucial roles in immune cell trafficking.
  • CCR8 is a chemokine receptor with a known homologue in humans.

Purpose of the Study:

  • To clone and characterize the mouse CCR8 gene.
  • To investigate the expression pattern of mouse CCR8.
  • To explore the potential role of CCR8 in Th2 immune responses.

Main Methods:

  • Gene cloning and characterization techniques.
  • Quantitative analysis of mRNA expression in various immune cells.
  • Comparison of murine and human CCR8 expression patterns.

Main Results:

  • Mouse CCR8 was successfully cloned and characterized.
  • CCR8 mRNA is predominantly expressed in the thymus.
  • Peripheral expression is highest in activated Th2-polarized cells and NK1.1+ CD4+ T cells.
  • Similar expression patterns were observed for human CCR8 in Th2 cells.
  • CCR8 ligands (I-309 and TCA-3) are chemoattractants for Th2 cells.

Conclusions:

  • CCR8 expression is associated with a Th2-specific gene expression program.
  • CCR8 likely plays a role in regulating Th2 immune responses.
  • CCR8 represents a potential therapeutic target for allergic diseases.

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