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Transcription01:10

Transcription

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Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
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Transcription01:10

Transcription

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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Transcription01:17

Transcription

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Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
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Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
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Transcription01:17

Transcription

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Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
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Integrar el procesamiento de ARNm con la transcripción.

Nick J Proudfoot1, Andre Furger, Michael J Dye

  • 1Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, United Kingdom. nicholas.proudfoot@path.ox.ac.uk

Cell
|March 23, 2002
PubMed
Resumen
Este resumen es generado por máquina.

Los eventos de procesamiento del ARN mensajero (ARNm) como el encapsulamiento, el empalme y la poliadenilación ocurren durante la transcripción. Estos procesos están coordinados por la transcripción e influyen unos en otros.

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Área de la Ciencia:

  • Biología Molecular Biología Molecular
  • Expresión génica de la expresión génica.
  • Procesamiento de ARN Procesamiento de ARN

Sus antecedentes:

  • El procesamiento del ARN mensajero (ARNm), incluido el encapsulamiento, el empalme y la poliadenilación, son pasos cruciales en la expresión génica.
  • Se sabe que estos eventos de procesamiento ocurren durante la transcripción de los genes.
  • La coordinación y la interacción entre estas reacciones no se comprenden completamente.

Objetivo del estudio:

  • Para aclarar la naturaleza cotranscripcional del procesamiento del ARNm.
  • Para investigar la coordinación entre el encapsulamiento, el empalme y la poliadenilación.
  • Comprender el papel de la ARN polimerasa II en la facilitación de estos procesos integrados.

Principales métodos:

  • Análisis de las vías de procesamiento del ARNm cotranscripcional.
  • Investigar las interacciones moleculares entre la transcripción y los factores de procesamiento de ARNm.
  • Estudiando la función del dominio C-terminal fosforilado (DTC) de la ARN polimerasa II.

Principales resultados:

  • El encapsulamiento, el empalme y la poliadenilación del ARNm ocurren simultáneamente con la transcripción.
  • Estas reacciones de procesamiento exhiben una influencia mutua en su eficiencia y especificidad.
  • La transcripción coordina activamente estos eventos de procesamiento de ARNm.
  • El CTD fosforilado de la ARN polimerasa II sirve como plataforma para las interacciones moleculares clave con los factores de procesamiento.

Conclusiones:

  • El procesamiento de ARNm es un proceso cotranscripcional integrado.
  • La transcripción juega un papel central en la coordinación de la tapa, el empalme y la poliadenilación del ARNm.
  • El CTD de la ARN polimerasa II es esencial para vincular la transcripción con la maduración del ARNm.