Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

Conjugated Proteins02:50

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Protein-protein Interfaces02:04

Protein-protein Interfaces

Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a polypeptide...
Cooperative Allosteric Transitions01:58

Cooperative Allosteric Transitions

Cooperative allosteric transitions can occur in multimeric proteins, where each subunit of the protein has its own ligand-binding site. When a ligand binds to any of these subunits, it triggers a conformational change that affects the binding sites in the other subunits; this can change the affinity of the other sites for their respective ligands. The ability of the protein to change the shape of its binding site is attributed to the presence of a mix of flexible and stable segments in the...
Protein Networks02:26

Protein Networks

An organism can have thousands of different proteins, and these proteins must cooperate to ensure the health of an organism. Proteins bind to other proteins and form complexes to carry out their functions. Many proteins interact with multiple other proteins creating a complex network of protein interactions.
These interactions can be represented through maps depicting protein-protein interaction networks, represented as nodes and edges. Nodes are circles that are representative of a protein,...
Conjugated Proteins02:50

Conjugated Proteins

Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
Protein Denaturation01:28

Protein Denaturation

The function of proteins depends on their native three-dimensional structure, which is dictated by the amino acid sequence of the specific protein. Folding of the polypeptide chain takes place under specific conditions that energetically favor the folded conformation. In contrast, protein denaturation occurs spontaneously under unfavorable conditions that disrupt the integrity of the folded conformation. Thus, the chemical and physical environment of a protein, such as significant changes in pH...

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

S-nitrosylation of protein kinase A is required for its activation by GPCRs.

bioRxiv : the preprint server for biology·2026
Same author

Nitric oxide drives proteomic diversity through alternative splicing.

Molecular cell·2026
Same author

SPARC is associated with tumor nerve stroma interactions and perineural invasion in pancreatic ductal adenocarcinoma.

Scientific reports·2026
Same author

[Ulcerative Colitis-Associated Cancer Resembling a Submucosal Tumor-A Case Report].

Gan to kagaku ryoho. Cancer & chemotherapy·2026
Same author

The protein denitrosylase SCoR2 regulates lipogenesis and fat storage.

Science signaling·2025
Same author

Lambert W Function in Solving Delay Differential Equations for Modeling in Economics and Finance.

Nonlinear dynamics, psychology, and life sciences·2025

Video Experimental Relacionado

Updated: Jul 7, 2026

Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study
12:43

Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study

Published on: July 27, 2016

Detección de las interacciones proteína-proteína dependientes del óxido nítrico.

Akio Matsumoto1, Karrie E Comatas, Limin Liu

  • 1Howard Hughes Medical Institute and Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA.

Science (New York, N.Y.)
|August 2, 2003
PubMed
Resumen

El óxido nítrico (NO) regula la señalización celular mediante la mediación de las interacciones proteína-proteína. Los investigadores identificaron interacciones dependientes de NO que involucran a la procaspasa-3, la esfingomielinasa ácida y la NO sintasa, lo que sugiere que la nitrosilación es un mecanismo regulador clave.

Más Videos Relacionados

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
10:24

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry

Published on: June 7, 2018

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
08:31

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

Videos de Experimentos Relacionados

Last Updated: Jul 7, 2026

Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study
12:43

Genetic and Biochemical Approaches for In Vivo and In Vitro Assessment of Protein Oligomerization: The Ryanodine Receptor Case Study

Published on: July 27, 2016

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
10:24

Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry

Published on: June 7, 2018

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
08:31

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

Área de la Ciencia:

  • La señalización celular de las células.
  • Biología molecular La biología molecular.
  • La bioquímica es la bioquímica.

Sus antecedentes:

  • El óxido nítrico (NO) es una molécula con diversas funciones en la señalización celular.
  • Las interacciones proteína-proteína son cruciales para las funciones celulares y las vías de señalización.
  • Comprender cómo el NO influye en estas interacciones es clave para descifrar la regulación celular.

Objetivo del estudio:

  • Para investigar el papel del óxido nítrico (NO) en la regulación de las interacciones proteína-proteína.
  • Identificar nuevos socios de unión NO-dependientes de la procaspasa-3 utilizando un sistema de dos híbridos de levadura modificada.

Principales métodos:

  • Modificación del sistema de dos híbridos de la levadura para detectar las interacciones NO-dependientes.
  • Cribado para socios de unión de la procaspasa-3.
  • Validación de las interacciones identificadas en células de mamíferos.

Principales resultados:

  • Se identificaron múltiples interacciones proteína-proteína dependientes del NO.
  • Se confirmaron interacciones específicas NO-dependientes entre la procaspasa-3, la esfingomielinasa ácida y la NO sintasa en células de mamíferos.
  • La evidencia sugiere que la nitrosilación es un mecanismo para regular las interacciones de las proteínas.

Conclusiones:

  • El óxido nítrico (NO) juega un papel importante en la modulación de las interacciones proteína-proteína.
  • La nitrosilación surge como un mecanismo potencialmente extendido para regular la formación de complejos proteicos.
  • Se necesitan más estudios proteómicos bajo condiciones redox controladas para descubrir interacciones adicionales mediadas por NO.