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Modelado y control del crecimiento por membrana atada Wingless Wingless.
Cyrille Alexandre1, Alberto Baena-Lopez1, Jean-Paul Vincent2
11] MRC National Institute for Medical Research, The Ridgeway, Mill Hill, London NW7 1AA, UK [2].
Nature
|January 7, 2014
Resumen
Las proteínas de señalización secretadas llamadas Wnts (sin alas) son esenciales para el desarrollo. Este estudio encontró que las Wnts no necesitan extenderse a los apéndices del patrón, desafiando suposiciones anteriores.
Área de la Ciencia:
- Biología del desarrollo Biología del desarrollo.
- La señalización celular de las células.
- Genética La genética.
Sus antecedentes:
- Las proteínas Wnt son moléculas de señalización secretadas y conservadas cruciales para el desarrollo embrionario.
- La señalización Wnt típicamente implica un gradiente formado por la propagación de proteínas Wnt desde su sitio de síntesis.
- La necesidad de la propagación de la proteína Wnt para el patrón de desarrollo no se ha verificado experimentalmente.
Objetivo del estudio:
- Investigar el requisito de la propagación de la proteína Wnt para el patrón de desarrollo y el crecimiento de apéndices.
- Para determinar si una variante Wnt no propagable puede apoyar el desarrollo normal.
Principales métodos:
- Ingeniería genómica en Drosophila para reemplazar el gen endógeno sin alas con una variante atada a la membrana.
- Análisis de la viabilidad de la mosca, el patrón de apéndice y el tamaño.
- Investigación de la expresión génica objetivo y los mecanismos de memoria de señalización.
Principales resultados:
- Las moscas que expresaban membrana atada sin alas eran viables con apéndices con patrones normales de tamaño casi normal, aunque el desarrollo se retrasó.
- La transcripción sin alas prolongada y la memoria de señalización permitieron la persistencia de la expresión génica objetivo en el ala en desarrollo.
- Se demostró que la propagación de la proteína sin alas es indispensable para el patrón y el crecimiento de los apéndices.
Conclusiones:
- La propagación de Wingless no es esencial para el patrón y el crecimiento de los apéndices de Drosophila.
- El patrón de desarrollo puede ocurrir a través de la señalización sostenida de una fuente localizada de Wnt que no se propaga.
- La memoria de señalización y la transcripción prolongada juegan un papel crítico para compensar la falta de propagación de Wnt.
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