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Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

9.4K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
9.4K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.3K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.3K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

11.7K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
11.7K
Scanning Electron Microscopy01:07

Scanning Electron Microscopy

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A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
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Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.2K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.0K

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Video Experimental Relacionado

Updated: May 1, 2026

High Content Screening in Neurodegenerative Diseases
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High Content Screening in Neurodegenerative Diseases

Published on: January 6, 2012

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Cribado de alto contenido basado en microscopía

Michael Boutros1, Florian Heigwer2, Christina Laufer2

  • 1Division Signaling and Functional Genomics, German Cancer Research Center (DKFZ) and Department of Cell and Molecular Biology, Heidelberg University, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany; German Cancer Consortium (DKTK), 69120 Heidelberg, Germany.

Cell
|December 7, 2015
PubMed
Resumen
Este resumen es generado por máquina.

El cribado basado en imágenes, combinado con perturbaciones, ofrece poderosas perspectivas sobre los procesos biológicos. Los avances en imágenes y análisis aceleran los estudios a gran escala, incluidas las aplicaciones de CRISPR.

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Área de la Ciencia:

  • * Biología celular y del organismo
  • * Cribado de alto contenido e imágenes
  • * Ingeniería genómica y genómica funcional

Sus antecedentes:

  • * El cribado basado en imágenes cuantifica los fenotipos en células y organismos.
  • * Las perturbaciones como la interferencia de ARN y las moléculas pequeñas mejoran las percepciones biológicas sistemáticas.
  • * Las aplicaciones abarcan la localización de proteínas, las vulnerabilidades al cáncer y los fenotipos de los organismos.

Objetivo del estudio:

  • * Revisar el estado de la técnica en la detección basada en imágenes.
  • * Delimitar los enfoques experimentales y de análisis de imágenes.
  • * Discutir los desafíos y las direcciones futuras, incluida CRISPR/Cas9.

Principales métodos:

  • * Utiliza el cribado basado en imágenes con varias perturbaciones (RNAi, moléculas pequeñas, mutaciones).
  • * Utiliza metodologías avanzadas de imagen y análisis de imágenes para pantallas a gran escala.
  • * Destaca la integración de la ingeniería genómica CRISPR/Cas9.

Principales resultados:

  • * El cribado basado en imágenes proporciona información sistemática sobre los procesos biológicos.
  • * Los avances recientes aceleran las pantallas de perturbación a gran escala.
  • * Se discuten las capacidades y limitaciones actuales de la tecnología.

Conclusiones:

  • El cribado basado en imágenes es una herramienta poderosa para el descubrimiento biológico.
  • * Los avances continuos en imágenes y análisis son cruciales.
  • * La integración CRISPR/Cas9 promete aplicaciones ampliadas.