Jove
Visualize
Contáctanos
JoVE
x logofacebook logolinkedin logoyoutube logo
ACERCA DE JoVE
Visión GeneralLiderazgoBlogCentro de Ayuda JoVE
AUTORES
Proceso de PublicaciónConsejo EditorialAlcance y PolíticasRevisión por ParesPreguntas FrecuentesEnviar
BIBLIOTECARIOS
TestimoniosSuscripcionesAccesoRecursosConsejo Asesor de BibliotecasPreguntas Frecuentes
INVESTIGACIÓN
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchivo
EDUCACIÓN
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualCentro de Recursos para ProfesoresSitio de Profesores
Términos y Condiciones de Uso
Política de Privacidad
Políticas

Videos de Conceptos Relacionados

MicroRNAs01:22

MicroRNAs

4.2K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
MicroRNAs01:22

MicroRNAs

24.5K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.5K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.5K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.5K
Master Transcription Regulators02:23

Master Transcription Regulators

8.0K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
8.0K
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

26.7K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
26.7K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.8K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.8K

También podría leer

Artículos Relacionados

Artículos vinculados a este trabajo por autores compartidos, revista y gráfico de citas.

Ordenar por
Same author

A conserved hormonal signalling-H2A.Z axis rapidly reorganizes 3D chromatin interactions in adipocyte thermogenesis.

Nature metabolism·2026
Same author

Hepatic glutathione depletion ameliorates MASLD through selective protein oxidation and inhibition of lipogenesis.

The Journal of clinical investigation·2026
Same author

Fatty acid regulation of feeding in <i>Caenorhabditis</i> elegans reveals the potential ancestral origin of a GLP-1-like multiagonist signaling system.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Response to Comment on Samovski et al. Effect of Marked Weight Loss on Adipose Tissue Biology in People With Obesity and Type 2 Diabetes. Diabetes Care 2025;48:1342-1351.

Diabetes care·2025
Same author

Portal vein-enriched metabolites as intermediate regulators of the gut microbiome in insulin resistance.

Cell metabolism·2025
Same author

Protein Kinase C δ: a critical hub regulating macrophage immunomodulatory functions during <i>Mycobacterium tuberculosis</i> infection.

bioRxiv : the preprint server for biology·2025

Video Experimental Relacionado

Updated: Mar 7, 2026

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
09:20

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

Published on: May 4, 2021

4.3K

Los miRNA circulantes derivados de la adiposa regulan la expresión génica en otros tejidos

Thomas Thomou1, Marcelo A Mori2, Jonathan M Dreyfuss3,4

  • 1Section on Integrative Physiology &Metabolism, Joslin Diabetes Center and Harvard Medical School, Boston, Massachusetts, USA.

Nature
|February 16, 2017
PubMed
Resumen

El tejido adiposo libera microARN exosomales (miRNA) que regulan el metabolismo. La restauración del tejido adiposo en ratones mejoró la tolerancia a la glucosa y redujo el FGF21, lo que sugiere que los miARN exosómicos actúan como nuevas adipocinas.

Más Videos Relacionados

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

8.7K
Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

3.0K

Videos de Experimentos Relacionados

Last Updated: Mar 7, 2026

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function
09:20

An Adipocyte Cell Culture Model to Study the Impact of Protein and Micro-RNA Modulation on Adipocyte Function

Published on: May 4, 2021

4.3K
Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

8.7K
Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator
06:08

Author Spotlight: Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

Published on: May 19, 2023

3.0K

Área de la Ciencia:

  • Regulación del metabolismo
  • Biología molecular
  • Endocrinología

Sus antecedentes:

  • El tejido adiposo es fundamental para la homeostasis energética y el control metabólico a través de las adipocinas.
  • Los microARN exosomales (miRNA) están implicados en la comunicación intercelular.
  • La desregulación del tejido adiposo está relacionada con trastornos metabólicos.

Objetivo del estudio:

  • Investigar el papel de los miARN exosómicos derivados del tejido adiposo en la regulación metabólica.
  • Para determinar si los miARN exosomales del tejido adiposo pueden actuar como moléculas de señalización.
  • Para explorar el potencial terapéutico de los exosomas derivados del tejido adiposo.

Principales métodos:

  • En ratones con Dicer knockout específico para el tejido adiposo (ADicerKO).
  • Se utilizaron datos de pacientes con lipodistrofia para la relevancia en humanos.
  • Realizó experimentos de trasplante de tejido adiposo.
  • Se administraron exosomas séricos normales y ADicerKO.
  • Valoración de la tolerancia a la glucosa y de la expresión hepática de Fgf21.
  • Se utilizan modelos de transferencia de miRNA in vivo.

Principales resultados:

  • Los ratones con ADicerKO y los pacientes con lipodistrofia mostraron una reducción de los miARN exosómicos circulantes.
  • El trasplante de tejido adiposo restauró los niveles de miARN circulantes y mejoró la tolerancia a la glucosa en ratones ADicerKO.
  • El trasplante también redujo el ARNm Fgf21 hepático y el FGF21 circulante.
  • Los exosomas normales del suero, pero no los exosomas de ADicerKO, imitaban estos efectos reguladores de genes.
  • La transferencia de miRNA in vivo demostró actividad de miRNA exosomal entre tejidos distantes.

Conclusiones:

  • El tejido adiposo es una fuente significativa de miRNAs exosómicos circulantes.
  • Estos miRNA exosómicos funcionan como moléculas de señalización, regulando la expresión génica en órganos distantes.
  • Los miARN exosomales representan una nueva clase de adipocinas con implicaciones terapéuticas potenciales para las enfermedades metabólicas.