Video Experimental Relacionado
Updated: Feb 13, 2026
08:31
Advancing High-Resolution Imaging of Virus Assemblies in Liquid and Ice
Published on: July 20, 2022
3.7K
Imagen de alta resolución del autoensamblaje coloidal de no equilibrio mediante fotofijación
Jagannath Satpathy1, Jim Jui-Kai Chen1, Gang Wen1,2
1Laboratory for Photochemistry and Spectroscopy, Division for Molecular Imaging and Photonics, Department of Chemistry, KU Leuven, Leuven 3001, Belgium.
ACS nano
|February 12, 2026
Resumen
Desarrollamos FRAME, un nuevo método para estudiar estructuras dinámicas de nanopartículas coloidales. Esta técnica utiliza luz UV para fijar estructuras, lo que permite un análisis detallado para diseñar materiales funcionales avanzados.
Área de la Ciencia:
- Ciencia coloidal
- Ciencia de materiales
- Nanotecnología
Sus antecedentes:
- Las nanopartículas coloidales se autoorganizan en estructuras de equilibrio y de no equilibrio.
- Los ensamblajes de no equilibrio son dinámicos y reconfigurables, lo que plantea desafíos para el análisis de la estructura-función.
- Los métodos de caracterización actuales luchan con la naturaleza transitoria de estos ensamblajes.
Objetivo del estudio:
- Presentar una metodología novedosa, Fijación y Resolución de Ensamble de Materia Activa Coloidal (FRAME), para caracterizar ensamblajes coloidales de no equilibrio.
- Permitir el análisis estructural detallado de estructuras coloidales dinámicas.
- Facilitar el diseño racional y la aplicación de materiales funcionales a partir de la autoorganización coloidal.
Principales métodos:
- Se desarrolló la metodología FRAME que combina la fotopolimerización UV para la fijación y la imagen de alta resolución (microscopía confocal 3D, SEM, 3D STED).
- Se aplicó FRAME a estructuras de Materia Óptica (OM) formadas en la interfaz de vidrio/agua utilizando una trampa óptica.
- Se analizaron estructuras compuestas de nanopartículas coloidales (200 nm a 1 μm).
Principales resultados:
- Se demostró que el proceso de fijación UV en FRAME no altera las propiedades estructurales de los ensamblajes coloidales.
- Se validó la precisión del análisis estructural utilizando técnicas de imagen de alta resolución después de la fijación.
- Se caracterizaron con éxito estructuras complejas de Materia Óptica con nanopartículas que van desde 200 nm hasta 1 μm.
Conclusiones:
- FRAME proporciona un método robusto y preciso para investigar ensamblajes coloidales de no equilibrio.
- Esta técnica supera las limitaciones de los métodos actuales para estudiar estructuras dinámicas transitorias.
- FRAME allana el camino para el diseño racional y la aplicación de materiales funcionales basados en la autoorganización coloidal activa.
Videos de Conceptos Relacionados
03:22Colloids
21.3K
Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles that are visible to the naked eye or can be seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. On the other hand, a solution is a homogeneous mixture in which no settling occurs and in which the dissolved...
21.3K
01:17Colloids and Suspensions
3.5K
Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles visible to the naked eye or seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. The suspended particles in a suspension settle out after some time of mixing. The separation of particles from a suspension is...
3.5K
01:09Colloidal precipitates
6.5K
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
6.5K
02:41Protein Complex Assembly
16.9K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types. Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.9K
02:50Spindle Assembly
4.3K
Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a...
4.3K
01:24Oligosaccharide Assembly
3.7K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
3.7K