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関連する概念動画

Establishment and Characterization of Patient-derived Gastric Organoids from Biopsies of Benign Gastric Body and Antral Epithelium08:45

Establishment and Characterization of Patient-derived Gastric Organoids from Biopsies of Benign Gastric Body and Antral Epithelium

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Gastric patient-derived organoids find increasing use in research, yet formal protocols for generating human gastric organoids from single-cell digests with standardized seeding density are lacking. This protocol presents a detailed method for reliably creating gastric organoids from biopsy tissue obtained during upper...
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Laparoscopy-endoscopy Cooperative Surgery for the Treatment of Gastric Gastrointestinal Stromal Tumors05:16

Laparoscopy-endoscopy Cooperative Surgery for the Treatment of Gastric Gastrointestinal Stromal Tumors

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Laparoscopy-endoscopy cooperative surgery is appropriate for the treatment of gastric gastrointestinal stromal tumors less than 5 cm in size. It can achieve the respective advantages of endoscopic and laparoscopic surgeries while avoiding their...
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Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization08:16

Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization

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Multiplex in situ hybridization (ISH) was employed to simultaneously visualize the transcripts for two G protein-coupled receptors and one transcription factor in the entire vagal ganglionic complex of the adult mouse. This protocol could be used to generate accurate maps of the transcriptional profiles of vagal afferent...
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One-anastomosis Gastric Bypass (OAGB) in Rats08:16

One-anastomosis Gastric Bypass (OAGB) in Rats

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This protocol is for the performance of one-anastomosis gastric bypass (OAGB) on rat. The operator performs a long and tubular-stapled gastric pouch followed by hand-sewn anastomosis. For this model, the operator reproduces the same ratio between biliopancreatic and common limb in humans; therefore, the biliopancreatic limb measures 35 cm.
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Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [13C]-octanoic Acid Breath Test12:24

Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [13C]-octanoic Acid Breath Test

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Determination of gastric emptying with a non-invasive [13C]-octanoic acid breath test for tracking gastroparesis in female NOD LtJ...
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Roux-en-Y Gastric Bypass Operation in Rats07:37

Roux-en-Y Gastric Bypass Operation in Rats

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Numerous studies using gastric bypass rat models have been recently conducted to uncover the underlying physiological mechanisms of Roux-en-Y gastric bypass operations. This article aims to demonstrate and discuss the technical and experimental details of our published gastric bypass rat model to understand advantages and limitations of this experimental...
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関連する実験動画

Updated: Feb 1, 2026

One-anastomosis Gastric Bypass OAGB in Rats
08:16

One-anastomosis Gastric Bypass OAGB in Rats

Published on: November 10, 2018

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膜区間の組織に関するゲノム学的視点

J B Bock1, H T Matern, A A Peden

  • 1Howard Hughes Medical Institute, Department of Molecular and Cellular Physiology, Stanford University School of Medicine, California 94305-5426, USA.

Nature
|March 10, 2001
PubMed
まとめ

研究者は,エウカリオット全体で膜組織に関与するタンパク質ファミリーを研究した. ラブ族は酵母からヒトへと大きく拡大し,SNAREタンパク質はヒトでは4つの異なるサブファミリーに分散した.

科学分野:

  • 分子生物学は分子生物学である.
  • ゲノミクスゲノミクスとは
  • 細胞生物学 細胞生物学

背景:

  • ユカリオットのゲノム配列決定は,タンパク質ファミリーの進化を研究する機会を提供します.
  • 膜区間の組織を理解することは,細胞生物学にとって極めて重要です.

研究 の 目的:

  • 多様な真核ゲノムにおける膜組織に関与する重要なタンパク質ファミリーの進化的拡大を調査する.
  • SNARE,コート複合体,Rab,Sec1タンパク質ファミリーのメンバーを特定し,分類する.

主な方法:

  • 4つのエウカリオトゲノム (酵母,ワーム,ハエ,ヒト) の比較ゲノミクス分析.
  • タンパク質ファミリーメンバーの識別と特徴付け.
  • タンパク質サブファミリーを解明するための系統遺伝分析.

主要な成果:

  • ラブタンパク質ファミリーは,酵母から多細胞生物への一貫した拡張を示しました.
  • 研究されたすべてのタンパク質ファミリー,特にSNAREs,Rabs,コート複合体のサブユニットは,ヒトにおいて有意に拡大した.
  • SNAREタンパク質ファミリーは,4つの異なるサブファミリーに分割されました.

さらに関連する動画

Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [13C]-octanoic Acid Breath Test
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Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [13C]-octanoic Acid Breath Test

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Roux-en-Y Gastric Bypass Operation in Rats
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Roux-en-Y Gastric Bypass Operation in Rats

Published on: June 11, 2012

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関連する実験動画

Last Updated: Feb 1, 2026

One-anastomosis Gastric Bypass OAGB in Rats
08:16

One-anastomosis Gastric Bypass OAGB in Rats

Published on: November 10, 2018

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Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [13C]-octanoic Acid Breath Test
12:24

Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [13C]-octanoic Acid Breath Test

Published on: March 23, 2013

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Roux-en-Y Gastric Bypass Operation in Rats
07:37

Roux-en-Y Gastric Bypass Operation in Rats

Published on: June 11, 2012

24.3K

結論:

  • タンパク質ファミリーの拡大,特にラブとSNAREは,真核細胞膜組織を形作る重要な進化的メカニズムです.
  • 人間の細胞は,膜の取引とコンパートメントの組織に関与するタンパク質の非常に広範なレパートリーを持っています.