Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Nucleic acids02:43

Nucleic acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Nucleic Acids02:43

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Mechanistic insights into Lin28-dependent oligo-uridylylation of pre-let-7 by TUT4.

Nucleic acids research·2026
Same author

Structures and mechanisms of U6 snRNA m<sup>6</sup>A modification by METTL16.

Nature communications·2025
Same author

Insights into G-protein coupling preference from cryo-EM structures of G<sub>q</sub>-bound PTH1R.

Nature chemical biology·2025
Same author

Cryo-EM structure of a blue-shifted channelrhodopsin from Klebsormidium nitens.

Nature communications·2025
Same author

Molecular fingerprints of a convergent mechanism orchestrating diverse ligand recognition and species-specific pharmacology at the complement anaphylatoxin receptors.

bioRxiv : the preprint server for biology·2025
Same author

Structure of a lasso peptide bound ET<sub>B</sub> receptor provides insights into the mechanism of GPCR inverse agonism.

Nature communications·2025

関連する実験動画

Updated: Jun 11, 2026

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

テンプレート独立RNAポリメリゼーションの構造的基礎

Kozo Tomita1, Shuya Fukai, Ryuichiro Ishitani

  • 1Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology, 1-1-1, Higashi, Tsukuba-shi, Ibaragi 305-8666, Japan.

Nature
|August 6, 2004
PubMed
まとめ

CCA添加酵素は,新しい方法で,必須の3'-端末移転RNA配列を合成する.

科学分野:

  • 分子生物学は分子生物学である.
  • 構造生物学 構造生物学とは
  • バイオケミストリー バイオケミストリー

背景:

  • 移転RNA (tRNA) の3'-末端CCA配列は,タンパク質合成に不可欠である.
  • CCA添加酵素,テンプレート独立RNAポリメラーゼは,CTPとATPを用いてこの配列を合成する.
  • テンプレート独立CCA合成のメカニズムは,未だに十分に理解されていない.

研究 の 目的:

  • CCA添加酵素によるテンプレート独立RNAポリメリゼーションのメカニズムを解明する.
  • ヌクレオチドの選択と添加に関する構造的な洞察を提供するためです.
  • 酵素が核酸テンプレートなしで配列特異性を達成する方法を理解する.

主な方法:

  • Aquifex aeolicusのCCA添加酵素のX線結晶学.
  • プライマーのtRNAとATPアナログとの共結晶化.
  • サイト・ディレクテッド・ミュータジェネシスと生化学分析.

主要な成果:

  • 2.8 Åの解像度でtRNAとATPアナログと結合するCCA添加酵素の結晶構造.
  • 酵素の触媒ポケットは"タンパク質テンプレート"を形成し,tRNAのC74-C75.5を認識する.

さらに関連する動画

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae
09:12

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae

Published on: February 27, 2026

Visualization and Quantification of Intermolecular RNA Base Pairing in in vitro RNA Clusters Using Split Broccoli RNA Reporters
10:52

Visualization and Quantification of Intermolecular RNA Base Pairing in in vitro RNA Clusters Using Split Broccoli RNA Reporters

Published on: May 29, 2026

関連する実験動画

Last Updated: Jun 11, 2026

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae
09:12

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae

Published on: February 27, 2026

Visualization and Quantification of Intermolecular RNA Base Pairing in in vitro RNA Clusters Using Split Broccoli RNA Reporters
10:52

Visualization and Quantification of Intermolecular RNA Base Pairing in in vitro RNA Clusters Using Split Broccoli RNA Reporters

Published on: May 29, 2026

  • 構造は,核酸選択の"挿入前"段階を明らかにしています.
  • ミュタゲネーシスの研究は,提案されたメカニズムを支持する.

    • 結論:

    • CCA添加酵素は,タンパク質テンプレートを使用して,3'-末端のCCA配列の合成を誘導します.
    • この構造的研究は,テンプレート独立のRNAポリメリゼーションのメカニズム的基礎を提供します.
    • この発見は,tRNAの成熟とタンパク質の合成に関する洞察を提供します.