2種類の異なる細胞のバイオコンパティブルなマイクロパターニング
Carlos C Co1, Yu-Chi Wang, Chia-Chi Ho
1Department of Chemical and Materials Engineering, University of Cincinnati, 497 Rhodes Hall, Cincinnati, OH 45221, USA.
Journal of the American Chemical Society
|February 11, 2005
まとめ
この研究は,複数の細胞タイプの非細胞毒性マイクロパターニングのための新しいポリエレクトロライト組立方法を導入しています. この技術は,生物分解性基板に内皮細胞と線維芽細胞を正確に配置することにより,複雑な組織工学を可能にします.
科学分野:
- バイオマテリアル科学 バイオマテリアル科学
- 組織工学は,組織工学である.
- 細胞生物学 細胞生物学
背景:
- ソフトリトグラフィーを用いたマイクロパターニング技術により,細胞の配置を制御できます.
- 既存の方法は,多細胞型パターニングの細胞抵抗性領域から細胞粘着性領域への非細胞毒性変換で課題に直面しています.
- 複雑な組織構造を in vitro で再現することは,組織工学の重要な障壁です.
研究 の 目的:
- 複数の細胞タイプの非細胞毒性マイクロパターニングのための新しいポリエレクトロライト組立アプローチを提示する.
- 生物分解性基板に異なる細胞タイプを連続的にパターン化する能力を実証する.
- 毛細血管ネットワークなどの組織化された細胞構造の創造におけるこの方法の応用を紹介する.
主な方法:
- 最初のマイクロパターニングのためにソフトリトグラフィーを利用しました.
- 細胞耐性領域を細胞粘着性領域に変換するために,ポリエレクトロライト組立技術を使用しました.
- 生物分解性基板の内皮細胞と線維芽細胞のパターンにこの方法を適用した.
- 内皮細胞によって誘発された毛細血管形成と,その後の線維芽細胞集合.
主要な成果:
- マイクロパターンの領域の非細胞毒性変換を成功裏に達成しました.
- 2つの異なる細胞タイプ (内皮細胞と線維芽細胞) の順次マイクロパターニングが成功していることが実証されました.
- 微細なパターンの線に沿った内皮細胞による組織化された毛細血管形成を展示した.
- パターン化された内皮細胞の周りの繊維芽細胞の後の集合を容易にした.
結論:
- ポリエレクトロライトアセンブリのアプローチは,多細胞型マイクロパターニングの汎用的で細胞毒性のないソリューションを提供します.
- この方法は,複雑な組織構造の in vitro 再構築のための組織工学の能力を向上させます.
- この技術は,血管組織工学を含む複数の細胞タイプの正確な空間制御を必要とするアプリケーションに期待されます.
さらに関連する動画
関連する概念動画
Cell Size
Cell sizes vary widely among and within organisms. Bacterial cells range between 1-10 micrometers (μm)and are considerably smaller than most eukaryotic cells. The smallest bacteria are 0.1 μm in diameter—about a thousand times smaller than eukaryotic cells, which typically range from 10-100 μm.Surface AreaCells can take in nutrients and water via diffusion through the plasma membrane itself or through specific channels in the membrane. The area of the membrane surrounding the cells limits the...
Cell Diversity
The concept of a cell started with microscopic observations of dead cork tissue by Robert Hooke in 1665. Hooke coined the term "cell" based on the resemblance of the small subdivisions in the cork to the rooms that monks inhabited, called cells. About ten years later, Antonie van Leeuwenhoek became the first person to observe the living and moving cells under a microscope. In the century that followed, the theory that cells represented the basic unit of life developed.
Multicellular organisms...
Multicellular organisms...
Capillaries and Their Types
Capillaries, a crucial constituent of the circulatory system, are diminutive vessels with a diameter between 5–10 micrometers, accommodating perfusion to the tissues through the phenomenon known as microcirculation. Through their permeable walls, consisting of an endothelial layer ensconced by a basement membrane and sporadically dispersed smooth muscle fibers, the exchange of substances between the blood and the interstitial fluid becomes plausible. Variance in wall composition exists, with...
Microbial Morphologies
Bacterial and archaeal cells exhibit remarkable diversity in shape and structure, critical in their adaptability and functionality. Among bacteria, the most commonly observed shapes include cocci and bacilli. Cocci are spherical and may exist singly or in groupings such as pairs (diplococci), chains (streptococci), clusters (staphylococci), or tetrads. Bacilli, in contrast, are rod-shaped and can also occur as single cells, in pairs, or chains, depending on their environmental and genetic...
Bacterial Phylum Tenericutes
The phylum Tenericutes, which includes the single class Mollicutes, comprises bacteria that lack cell walls. The term "Mollicutes" derives from the Latin word mollis, meaning "soft." These organisms are among the smallest known and are commonly referred to as mycoplasmas due to the prominence of the genus Mycoplasma, which includes well-known human pathogens. Despite their inability to stain gram-positively (a result of their lack of cell walls), mycoplasmas are phylogenetically related to the...
Diversity of Protists II
Alveolates are a group of organisms recognized by the presence of alveoli, which are cytoplasmic sacs located beneath the cell membrane. While their function remains uncertain, alveoli may help regulate water balance by controlling how much water enters and leaves the cell. In dinoflagellates, these structures may serve as armor plates. There are three major types of alveolates: ciliates, which move using cilia; dinoflagellates, which use flagella for movement; and apicomplexans, which are...


