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タンパク質相関プロファイリングによる哺乳類の器官図
Leonard J Foster1, Carmen L de Hoog, Yanling Zhang
1Center for Experimental BioInformatics (CEBI), Department of Biochemistry and Molecular Biology, University of Southern Denmark, Campusvej 55, DK-5230 Odense M, Denmark.
Cell
|April 18, 2006
まとめ
研究者は,タンパク質相関プロファイリングを使用して,マウス肝臓の10つのサブセルラー部位に1,404のタンパク質をマッピングしました. この研究は,臓器タンパク質の39%の複数の位置を明らかにし,臓器分析のために細胞生物学とゲノミクスを統合しました.
科学分野:
- 細胞生物学 細胞生物学
- プロテオミクス プロテオミクスは,プロテオミクスの
- ゲノミクスゲノミクスとは
背景:
- タンパク質の局所化は,細胞組織にとって極めて重要です.
- 臓器細胞タンパク質の理解は,細胞生物学にとって不可欠である.
- 既存のプロテオミックデータは,検証と精錬が必要である.
研究 の 目的:
- ネズミの肝臓の10つのサブセルラー部位にタンパク質の局所化をマッピングする.
- 公開されたオルゲンラー・プロテオミック・インベントリの特異性を評価する.
- プロテオミクとゲノミクデータを統合して,臓器生物発生の洞察を得ること.
主な方法:
- タンパク質相関プロファイリングは,タンパク質の局所化をマッピングするために使用されました.
- 酵素測定法,マーカータンパク質プロファイル,およびコンフォカル顕微鏡が検証に使用されました.
- プロテオミックとゲノムデータの統合が行われました.
主要な成果:
- 1,404のタンパク質がマウスの肝臓の10のサブセルラー部位にマッピングされました.
- 全臓器タンパク質の39%で複数の位置が特定されました.
- 共同発現する遺伝子,シス調節モチーフ,およびオルゲネルの生殖に関与する転写レギュレータのネットワークが特定されました.
結論:
- この研究は,マウス肝臓の臓器細胞におけるタンパク質の局所化の包括的な地図を提供します.
- 有機細胞タンパク質の有意な割合は,複数の細胞下部部位を示しています.
- マルチオミックスのデータの統合は,臓器生物発生と機能を研究するための統一された枠組みを提供します.
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