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関連する概念動画

COP Coated Vesicles00:59

COP Coated Vesicles

18.7K
Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
18.7K
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

4.4K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
4.4K
Coat Assembly and GTPases01:33

Coat Assembly and GTPases

4.7K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
4.7K
Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

3.4K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
3.4K
Transport Across the Golgi01:26

Transport Across the Golgi

6.7K
While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
6.7K
Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

10.2K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
10.2K

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関連する実験動画

Updated: Apr 8, 2026

In vivo and in vitro Studies of Adaptor-clathrin Interaction
17:14

In vivo and in vitro Studies of Adaptor-clathrin Interaction

Published on: January 27, 2011

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貨物はクラトリンでコーティングされたピットダイナミクスを調節する.

Manojkumar A Puthenveedu1, Mark von Zastrow

  • 1Department of Psychiatry, University of California at San Francisco, 600 16th Street, San Francisco, CA 94158, USA.

Cell
|October 5, 2006
PubMed
まとめ
この要約は機械生成です。

Gタンパク質結合受容体 (GPCRs) の調節された内細胞化は,クラトリンコーティングピット (CCP) の特定のサブセットを使用します. このCCPの貨物特有の規制は,内細胞経路における機能的専門化を可能にします.

さらに関連する動画

Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
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Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

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Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
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Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast

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関連する実験動画

Last Updated: Apr 8, 2026

In vivo and in vitro Studies of Adaptor-clathrin Interaction
17:14

In vivo and in vitro Studies of Adaptor-clathrin Interaction

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Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy
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Visualizing Clathrin-mediated Endocytosis of G Protein-coupled Receptors at Single-event Resolution via TIRF Microscopy

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Applications of pHluorin for Quantitative, Kinetic and High-throughput Analysis of Endocytosis in Budding Yeast
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科学分野:

  • 細胞生物学 細胞生物学
  • 分子生物学は分子生物学である.
  • バイオケミストリー バイオケミストリー

背景:

  • クラトリンコーティングピット (CCP) は,鍵となる内細胞機構である.
  • CCPは,通常,多様な膜貨物を扱う均一な集団と見なされます.
  • 貨物の分離とCCPのダイナミクスを支配する特定のメカニズムは,まだ完全に理解されていません.

研究 の 目的:

  • Gタンパク質結合受容体 (GPCRs) のような特定の貨物のCCPサブセットが,エンドサイトーシスのために異なるCCPサブセットを使用するかどうかを調査する.
  • GPCRを含むCCPがユニークな機能特性を有するかどうかを判断する.
  • 貨物の内容に基づいて,CCPのダイナミクスを制御する規制メカニズムを明らかにする.

主な方法:

  • CCPの動態と貨物取引を観察するための生細胞画像撮影.
  • PDZドメイン媒介のリンクを含む,タンパク質-タンパク質の相互作用を分析するための生化学的分析.
  • CCPの組立と分解に対する貨物の影響を評価するための混乱調査.

主要な成果:

  • GPCRsの調節されたエンドサイトーシスは,好ましくは,CCPsの特殊なサブセットを通じて発生します.
  • GPCRを含むCCPは,貨物-アクチン細胞骨格の相互作用によって調節される長時間の表面滞在時間を示す.
  • これらの専門的なCPCは,ダイナミンの徴用が遅れていることを示し,コートの解体と貨物の内部化を切り離すような失敗的な出来事を経験することがあります.

結論:

  • GPCRなどの内細胞性貨物は,異なるCCPサブセットに分割することができます.
  • CCPダイナミクスの貨物依存的調節は,機能的専門化のための運動的メカニズムを提供します.
  • この専門化は,初期の内細胞経路を最適化し,異なる貨物タイプの間の競争を緩和します.