多層のDNAオリガミが四角格子状の格子に詰め込まれています
Yonggang Ke1, Shawn M Douglas, Minghui Liu
1Department of Chemistry and Biochemistry, and the Biodesign Institute, Arizona State University, Tempe, Arizona 85287, USA.
Journal of the American Chemical Society
|October 8, 2009
まとめ
研究者らは,正方形の格子を使ってコンパクトな3D DNA オリガミデザインを開発した. この進歩により,DNAナノテクノロジーの精度と汎用性が向上した複雑なナノスケール形状の作成が可能になります.
科学分野:
- ナノテクノロジー ナノテクノロジー
- 分子生物学は分子生物学である.
- マテリアルサイエンス 材料科学
背景:
- DNAの自己組み立ては,ナノスケールの構造を作成するための強力な方法です.
- 折りたたまれたDNAオリガミは,長いDNA鎖を折りたたんでカスタム形にすることができます.
- 以前の3D DNA オリガミでは,プレートヘリックス層にハネコブの格子を使用していました.
研究 の 目的:
- 正方形の格子に基づいたよりコンパクトな3D DNA オリガミデザインを導入します.
- ヘリックス密度が増加した3D構造の成功折り畳みを実証する.
- DNAナノテクノロジーの汎用性と範囲を高めること.
主な方法:
- DNAヘリクスの方形格子配列を用いた.
- 構造の折りたたみのために1段階の退火プロセスを採用しました.
- 制御された寸法を持つカスタム 3D 形状を設計します.
主要な成果:
- 3D DNA オリガミの構造を正方形の格子で折り畳むことに成功しました.
- 以前の方法と比較してヘリックスパッキング密度の増加が実証されました.
- ナノスケールで精密に測定されたオブジェクトを作成しました.
結論:
- スクウェア・ラティス 3D DNA オリガミデザインは,よりコンパクトで汎用的なプラットフォームを提供します.
- この方法は,より平らな表面と長方形の構造の作成を容易にする.
- DNAナノテクノロジーアプリケーションの基礎的な進歩を表しています.
関連する概念動画
00:58DNA Packaging
Overview
00:58DNA Packaging
Overview
00:58Genomic DNA in Eukaryotes
Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
01:32Chromatin Packaging
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
02:21Chromatin Packaging
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter?
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order structures.
02:05DNA as a Genetic Template
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...