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Updated: Jun 12, 2026

Site Directed Spin Labeling and EPR Spectroscopic Studies of Pentameric Ligand-Gated Ion Channels
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Gd3+スピンラベリングを用いたタンパク質のナノメートルスケールの距離測定

Alexey Potapov1, Hiromasa Yagi, Thomas Huber

  • 1Department of Chemical Physics, Weizmann Institute of Science, Rehovot, 76100, Israel.

Journal of the American Chemical Society
|June 12, 2010
PubMed
まとめ

高場電子パラマグネティック共振 (EPR) を使用してタンパク質のナノメートル距離を測定するためのガドリニウム (((3+)) (Gd3+) スピンラベルを導入します. Gd3+ラベリングは感度が向上し,指向選択の問題が回避され,タンパク質構造分析の有効な技術となります.

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...