Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Nitric Oxide Signaling Pathway01:28

Nitric Oxide Signaling Pathway

Nitric oxide (NO), an inorganic gas, acts as a potent second messenger in most animal and plant tissues. NO diffuses out of the cells that produce it and enters the neighboring cells to generate a downstream response. NO synthase (NOS) catalyzes NO production by the deamination of the amino acid arginine. There are three isoforms of NOS. Endothelial cells have endothelial NOS (eNOS), nerve and muscle cells have neuronal NOS (nNOS), and macrophages produce inducible NOS (iNOS) upon exposure to...
Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
Antihypertensive Drugs: Vasodilators01:23

Antihypertensive Drugs: Vasodilators

Vasodilators, primarily affecting the smooth muscles within arterial and venous walls, are commonly used for hypertension treatment. Medications such as minoxidil and hydralazine primarily target arteries and arterioles, while sodium nitroprusside acts on arterioles and venules. Minoxidil, functioning as a prodrug, is metabolized by hepatic sulfotransferase into its active form, minoxidil sulfate, after oral administration. This metabolite binds to the sulfonylurea receptor (SUR) component of...
Activation and Inactivation of G Proteins01:22

Activation and Inactivation of G Proteins

Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high affinity and are together...
GPCR Desensitization01:12

GPCR Desensitization

G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
G-Protein Gated Ion Channels01:21

G-Protein Gated Ion Channels

GPCRs are primarily responsible for our sense of smell, taste, and vision.  The binding of a sensory stimulus activates GPCR to stimulate effector proteins, many of which are ion channels in the sensory organs. GPCRs modulate the opening and closing of the target ion channels either directly by binding them, or by releasing second messengers that activate these channels. As ions move across the membrane, the membrane potential is altered, which induces an appropriate response.
Sensory organs,...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Multistep electron tunneling through tryptophans in the KatG bifunctional peroxidase monitored by a nonperturbing spin probe.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Next generation protein-corrole bio-assemblies provide effective tumoricidal treatment in a metastatic triple-negative breast cancer model.

bioRxiv : the preprint server for biology·2026
Same author

Electron Transport through a Tryptophan Quadruplex in a Dimeric Azurin Construct.

The journal of physical chemistry. B·2026
Same author

Cooperative ligand binding in a bacterial heme-based oxygen sensor.

The Journal of biological chemistry·2025
Same author

Oxidizable amino acids around cytochrome P450 hemes.

Journal of inorganic biochemistry·2025
Same author

Roles of biological heme-based sensors of O<sub>2</sub> in controlling bacterial behavior.

Journal of inorganic biochemistry·2025

関連する実験動画

Updated: May 26, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO&#8226;) Probes, the geNOps, for Real-time Imaging of NO&#8226; Signals in Single Cells
08:32

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

ゲッティングは,酸化窒素合成酵素からNOが放出される.

Charlotte A Whited1, Jeffrey J Warren, Katherine D Lavoie

  • 1Beckman Institute and Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, California 91125, USA.

Journal of the American Chemical Society
|December 14, 2011
PubMed
まとめ

私たちは,Geobacillus stearothermophilus nitric oxide synthase (gsNOS) から排出される酸化窒素 (NO) を研究しました. 特定の場所の変異は,位置223と134の両方がゲートとして機能し,NOの脱出を制御することを明らかにしました.

さらに関連する動画

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
08:58

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

Measurement of Cyclic Guanosine Monophosphate (cGMP) in Solid Tissues using Competitive Enzyme-Linked Immunosorbent Assay (ELISA)
07:15

Measurement of Cyclic Guanosine Monophosphate (cGMP) in Solid Tissues using Competitive Enzyme-Linked Immunosorbent Assay (ELISA)

Published on: July 3, 2025

関連する実験動画

Last Updated: May 26, 2026

Application of Genetically Encoded Fluorescent Nitric Oxide (NO&#8226;) Probes, the geNOps, for Real-time Imaging of NO&#8226; Signals in Single Cells
08:32

Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells

Published on: March 16, 2017

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
08:58

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries

Published on: February 25, 2016

Measurement of Cyclic Guanosine Monophosphate (cGMP) in Solid Tissues using Competitive Enzyme-Linked Immunosorbent Assay (ELISA)
07:15

Measurement of Cyclic Guanosine Monophosphate (cGMP) in Solid Tissues using Competitive Enzyme-Linked Immunosorbent Assay (ELISA)

Published on: July 3, 2025

科学分野:

  • バイオケミストリー バイオケミストリー
  • 酵素学 酵素学とは
  • 分子生物学は分子生物学である.

背景:

  • 酸化窒素合成酵素 (NOS) は,生物系の重要な酵素である.
  • 以前の研究では,哺乳類のNOS.で酸化窒素 (NO) の放出のためのゲートメカニズムが示唆されました.
  • 細菌のNOSの構造と機能は,Geobacillus stearothermophilus NOS (gsNOS) のように,NO放出運動に関して,あまり理解されていません.

研究 の 目的:

  • 野生型gsNOSから脱出するNOの運動性を調査する.
  • gsNOS.から NO の放出を調節する特定のアミノ酸残留 (位置223と134) の役割を決定する.
  • 既知の哺乳類のNOS酵素とgsNOSのNO放出率を比較する.

主な方法:

  • 反応をモニタリングするために,ストップフローUV-VISスペクトロスコピーを使用しました.
  • 野生型のgsNOSと特定の変異種 (H134S,I223V,H134S/I223V) に関する運動実験が行われました.
  • 反応は,還元された酵素/N-ヒドロキシ-l-アルギニン複合体と空気性バッファを混合することによって開始されました.

主要な成果:

  • 野生型のgsNOSは,特徴づけられたNOS酵素の中で最も遅いNO放出率を示しています.
  • 位置223と134での変異は,NOの脱出率を大幅に増加させた.
  • 双重変異種 (H134S/I223V) は,哺乳類のNOS酵素の中で最も速いNOS酵素と同等のNO放出率を示した.
  • 位置223と134でのステリック阻害は,NOの放出を阻害する重要な要因として特定されました.

結論:

  • gsNOSの位置223と134は,NOの放出を制御する重要なゲートとして機能しています.
  • これらのゲート残基を調節することで,NOの放出運動を劇的に変化させることができます.
  • これらのゲーティングメカニズムの理解は,NOS酵素の機能と異なる種間の調節に関する洞察を提供します.