オーケストラによるイントロン保持は,正常な粒細胞の分化を調節する.
Justin J-L Wong1, William Ritchie, Olivia A Ebner
1Gene and Stem Cell Therapy Program, Centenary Institute, Camperdown 2050, Australia.
Cell
|August 6, 2013
まとめ
イントロン保持 (IR) は,単にミススプライシングではなく,正常な遺伝子発現制御メカニズムです. この保存された過程は,ナンセンス媒介性衰退 (NMD) と組み合わせて,粒子の形成の間に遺伝子発現を調節します.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝子規制 遺伝子規制
- 細胞の微分化は
背景:
- イントロン保持 (IR) は通常,スプライシングエラーとして見られます.
- 正常な生理学的プロセスにおけるその役割は十分に理解されていません.
研究 の 目的:
- 白血球の分化過程における正常な遺伝子発現におけるイントロン保持の役割を調査する.
- IRが規制されたメカニズムであり,それに関連する経路であるかどうかを判断する.
主な方法:
- 通常の白血球の分化によるトランスクリプトミクスとプロテオミクスデータのバイオ情報分析.
- 遺伝子発現の調節,スプライシング因子,GC含有量,ヒトとマウスの間の保存経路を調査した.
主要な成果:
- 粒子の形成過程で,機能的に関連した86の遺伝子の遺伝子発現を制御する生理学的メカニズムとして,イントロン保持 (IR) を特定した.
- 人とマウスの体内で保存されるIRは,ナンセンス媒介衰退 (NMD) 経路を通じてmRNAとタンパク質のレベルを低下させることを実証した.
- IRとスプライシングファクターのダウンレギュレーションおよび高GC含有量との関連が観察されました.
結論:
- 無意味な媒介による衰退と組み合わせたイントロン保持は,正常な粒子の形成における遺伝子発現制御のための保存された生理学的メカニズムです.
- この調節されたIRは,持続的な翻訳の前に,ダイナミックな遺伝子発現制御のためのエネルギー的に有利な方法を提供します.
関連する概念動画
Differentiation of Common Myeloid Progenitor Cells
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
Exon Recombination
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Chromatin Structure Regulates pre-mRNA Processing
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
Lineage Commitment
Commitment is the process whereby stem cells:
Inheritance of Chromatin Structures
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying DNA...
Regulation of Hematopoietic Stem Cells
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...


