Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

8.9K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
8.9K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

3.0K
3.0K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

17.2K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
17.2K
Eukaryotic RNA Polymerases00:58

Eukaryotic RNA Polymerases

8.4K
8.4K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

6.6K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
6.6K
Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

14.2K
In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
14.2K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Molecular basis of polyadenylated RNA fate determination in the nucleus.

Nature·2026
Same author

Mechanism of RACK1-dependent ZAKα activation at stalled and collided ribosomes.

Molecular cell·2026
Same author

A kinetic ruler controls mRNA poly(A) tail length.

Genes & development·2025
Same author

Structural basis for the synergistic assembly of the snRNA export complex.

Nature structural & molecular biology·2025
Same author

Integrator: A guardian against RNA-induced chaos.

Cell chemical biology·2025
Same author

True length of diverse capped RNA sequencing (TLDR-seq): 5'-3'-end sequencing of capped RNAs regardless of 3'-end status.

Nucleic acids research·2025
Same journal

Co-option of lysosomal machinery shapes the evolution of the intracellular photosymbiosis supporting coral reefs.

Cell·2026
Same journal

LEF1 and niche factors determine T cell stemness across chronic diseases.

Cell·2026
Same journal

Recurrent patterns of TOP1-mediated neuronal genomic damage shared by major neurodegenerative disorders.

Cell·2026
Same journal

Four-dimensional molecular mapping from a spatial snapshot reveals the dynamics of hair follicle organogenesis.

Cell·2026
Same journal

Whole-cell particle-based digital twin simulations from 4D lattice light-sheet microscopy data.

Cell·2026
Same journal

Systematic discovery of pathogen effector functions across human pathogens and pathways.

Cell·2026
関連記事をすべて見る

関連する実験動画

Updated: Apr 29, 2026

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

9.2K

スナップショット:核RNAPIIのトランスクリプト修正

Manfred Schmid1, Torben Heick Jensen1

  • 1Centre for mRNP Biogenesis and Metabolism, Department of Molecular Biology and Genetics, Aarhus University, 8000 Aarhus, Denmark.

Cell
|May 27, 2014
PubMed
まとめ
この要約は機械生成です。

RNAポリメラーゼIIは,mRNAやmiRNAなどの様々なRNA分子を生成する. これらの重要なトランスクリプトは,特定の酵素によって促進され,細胞核内で重要な改変と処理を受けます.

さらに関連する動画

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity
07:46

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

Published on: October 8, 2018

6.2K
Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
09:21

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Published on: October 22, 2018

8.6K

関連する実験動画

Last Updated: Apr 29, 2026

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
10:59

Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events

Published on: May 13, 2019

9.2K
An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity
07:46

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

Published on: October 8, 2018

6.2K
Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
09:21

Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity

Published on: October 22, 2018

8.6K

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • バイオケミストリー バイオケミストリー

背景:

  • RNAポリメラーゼIIは遺伝子発現の中心であり,様々な種類のRNAを生成します.
  • 新生RNAのトランスクリプトは,広範囲にわたる転写後の改変を必要とします.
  • 核酵素は,RNAの処理と成熟において重要な役割を果たします.

研究 の 目的:

  • RNA処理経路の簡潔な概要を提供するために.
  • 核RNAの改変に関与する酵素を強調する.
  • RNAポリメラーゼIIによって生成されるRNAトランスクリプトの多様性を説明します.

主な方法:

  • 文献レビューと現在の研究の合成.
  • RNA処理ステップの視覚表現 (スナップショット形式).
  • 主要な酵素とその機能に注目してください.

主要な成果:

  • mRNA,miRNA,lncRNA,およびsn(o) RNAの処理の詳細な描写.
  • 核RNA改変のための必須酵素の特定.
  • RNA成熟の連続的なステップの明確化.

結論:

  • RNAポリメラーゼIIの転写は,複雑なRNA処理カスケードの第一段階です.
  • 核酵素は,機能的なRNA分子を生成するために不可欠です.
  • これらのプロセスを理解することは,遺伝子調節と細胞機能を理解するために不可欠です.