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Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

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DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
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Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

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The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
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Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

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Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

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For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Homologous Recombination02:31

Homologous Recombination

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Updated: Apr 20, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
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Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes

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複数のデュプレックスストームを単一の四重複ループで結合する.

Kah Wai Lim1, Thi Quynh Ngoc Nguyen, Anh Tuân Phan

  • 1School of Physical and Mathematical Sciences, Nanyang Technological University , 637371 Singapore.

Journal of the American Chemical Society
|December 3, 2014
PubMed
まとめ

研究者らは,複数の茎を持つ安定したDNA四重複複複複合複合複合複合体を生み出した. このブレークスルーにより,新しいDNAアーキテクチャとゲノム応用のための潜在的な新薬標的が可能になります.

科学分野:

  • 分子生物学は分子生物学である.
  • 構造生物学 構造生物学とは
  • バイオケミストリー バイオケミストリー

背景:

  • DNAは,正規のダブルヘリックスを超えて複雑な非正規の構造を形成することができます.
  • G濃度の高い配列からなる四重複構造は,生物学的役割としてますます認識されています.
  • 複雑なDNAアセンブリの形成と性質を理解することは,ナノテクノロジーと医学にとって極めて重要です.

研究 の 目的:

  • 安定したDNAの四重複複複複合複合複合複合複合複合複合複合複合複合複合複合複合複合複合複合複合複合複合複合複合複合.
  • 単一の四重回路の中で複数の二重回路を組み込む原理を実証する.
  • 多様なDNAアーキテクチャと新たな治療目標の設計の可能性を探求する.

主な方法:

  • 四重複二重複のハイブリッドを形成するように設計されたDNA構造の合成.
  • 折り畳みトポロジーと構造的整合性を検証するための核磁気共鳴 (NMR) スペクトロスコーピー.
  • マルチスティム組み込み原理を評価するために,ループの位置と茎の数を体系的に変化させる.

主要な成果:

  • 安定したDNA四重複-二重複のハイブリッド複合体の形成に成功した.

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Studying DNA Looping by Single-Molecule FRET
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Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
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関連する実験動画

Last Updated: Apr 20, 2026

Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
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Studying DNA Looping by Single-Molecule FRET
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Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
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  • NMRスペクトロスコーピーを用いた2本の幹の四重複複複複複合混合構造の検証.
  • マルチステム組み込み原理が多用途で,より多くのステムと異なるループ位置に拡張できることを実証.

    • 結論:

    • 多重複素幹を四重複素ループに統合することによって複雑なDNAアーキテクチャを構築するための新しい原理が確立されました.
    • これらの多幹DNA複合体は,DNAナノテクノロジーの新しい設計戦略を提供します.
    • この発見は,ゲノム配列における潜在的な生物学的関連性を示唆し,これらの複雑なモチーフを標的とした薬の開発のための新しい道を示しています.