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Updated: Jul 30, 2026

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
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クロマチンのアクセシビリティの複合単細胞プロファイリングは,結合細胞インデックスによる

Darren A Cusanovich1, Riza Daza1, Andrew Adey2

  • 1University of Washington, Department of Genome Sciences, Seattle, WA, USA.

Science (New York, N.Y.)
|May 9, 2015
PubMed
まとめ

この研究は,何千もの単細胞のエピゲノムプロファイルのスケーラブルな方法を導入し,以前の制限を克服しました. この新しい技術は,細胞アトラスプロジェクトを進めるために,詳細なクロマチンのアクセシビリティマッピングを可能にします.

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Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...