CRISPRによるプログラム可能な3Dゲノム位置付けと核組織
Haifeng Wang1, Xiaoshu Xu1, Cindy M Nguyen1
1Department of Bioengineering, Stanford University, Stanford, CA 94305, USA.
Cell
|October 16, 2018
まとめ
研究 者 たち は,DNA を 制御 する CRISPR-ゲノム 機構 (CRISPR-GO) を 開発 し た
科学分野:
- 細胞生物学
- 遺伝学
- 分子生物学
背景:
- 空間的なゲノム組織は遺伝子調節と細胞機能に影響します
- クロマチンと核の相互作用を理解することは極めて重要です.
- 既存の方法では,ゲノム位置を正確に制御することができない.
研究 の 目的:
- 空間的なゲノム組織を制御するためのプログラム可能なシステムを開発する.
- クロマチンと核の間のダイナミックな関係を調査する.
- ゲノムポジショニングが遺伝子発現に与える影響を調査する.
主な方法:
- 化学的に誘導され,逆戻り可能なCRISPRゲノム組織 (CRISPR-GO) システムの開発.
- CRISPR-GOを使用して,核コンパートメント (例えば,核周辺部,カジャル体,PML体) に関するゲノム位置づけを操作する.
- 生体細胞におけるクロマチンの相互作用と遺伝子発現のリアルタイムモニタリング
主要な成果:
- CRISPR-GOはゲノム位置を 効率的かつプログラム可能な方法で制御します
- 核の周辺部への誘導可能な位置転換を証明し,ミトーシス依存およびミトーシス依存のイベントを解剖した.
- CRISPR-GOがカジャル体の形成を誘発し 長い距離で遺伝子の発現を抑制する能力を示しました
結論:
- CRISPR-GOは空間的なゲノム組織と機能を研究するための 多様性のあるプラットフォームを提供します
- このシステムは,クロマチンの動態と遺伝子調節のリアルタイムの調査を可能にする.
- プログラム可能なゲノム組織制御は 核の構造と機能の関係を理解するための新しい道を提供します
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