ホストで生成されたクオラムセンシング自動誘発器は,ファージ溶解-溶解決定を制御する
Justin E Silpe1, Bonnie L Bassler2
1Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA.
Cell
|December 18, 2018
まとめ
ビブリオ・コレラとそのファージは 同じ信号分子であるDPOを 病原化のために使用します フェーグVqmAは宿主の毒性遺伝子を活性化し,宿主VqmAはそれらを抑制し,細菌感染における複雑な相互作用を明らかにします.
科学分野:
- 微生物学
- 分子生物学
- 細菌学
背景:
- Vibrio choleraeは,3,5-dimethylpyrazin-2-ol (DPO) とVqmA (VqmAVc) を含むクオラムセンシング (QS) システムを利用して,毒性とバイオフィルム形成を調節する.
- vqmA遺伝子は,ビブリオ種とビブリオファージVP882で発見され,水平遺伝子の移転と共通の規制メカニズムを示唆しています.
研究 の 目的:
- V. コレラと感染するファグの相互作用におけるファグでコードされたVqmA (VqmAPhage) の役割を調査する.
- ファージが宿主由来信号を生命周期決定にどのように統合するか (溶解-溶生成) を理解する.
- 細菌の感染を制御するファグの再プログラムの可能性を探るため
主な方法:
- DPOとのVqmAVcとVqmAPhageの相互作用を比較した分析
- ファグ抗抑制剤の機能と関連するファグ抑制剤との干渉の調査.
- 宿主VqmAVcレギュロンのVqmAファージによる活性化を評価するための機能検査.
- 溶解誘導のためのユーザー定義のシグナルに反応するようにファージを再プログラムする.
主要な成果:
- ファージVqmAファージは宿主生成のDPOに結合し,ファージ解離プログラムを開始する.
- VqmAPhageは,ファグ抑制剤を無効化し,関連するファグ抑制剤に干渉する抗抑制剤を介して作用します.
- VqmAPhageは宿主VqmAVcレギュロンを活性化させ,非対称な調節相互作用を示している.
- ホストVqmAVcは,ファグ媒介による溶解を誘導することができず,単方向のファグの影響を強調する.
結論:
- V. コレラと感染ファージは,同じ信号分子 (DPO) に依存しています.
- ファージは宿主QS機構を自身の伝播のために利用し,宿主信号を溶解-溶解決定に統合する.
- 細菌菌を再プログラムすることで 細菌感染症に対する 治療的介入の戦略が生まれます
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