RNAのクロスキラル,RNA触媒による指数関数増幅
Grant A L Bare1, Gerald F Joyce1
1The Salk Institute, 10010 North Torrey Pines Road, La Jolla, California 92037, United States.
Journal of the American Chemical Society
|November 3, 2021
まとめ
この研究では,RNA分子のクロスキラル指数関数増幅が示され,逆向きのRNA酵素の生成と進化を可能にします. この突破は 生命の起源と プレバイオティック化学の可能性を広げています
科学分野:
- 生物化学
- 生命 研究 の 起源
- 分子生物学
背景:
- 生物学的情報マクロ分子にはキラル均一性がある (核酸のd核酸,タンパク質のlアミノ酸).
- この均一性は,潜在的に都合が良いが,化学的な必要条件ではない.
- 以前の研究では 逆キラリティの RNA 分子を増幅する方法がなかった.
研究 の 目的:
- RNAのクロスキラル指数関数増幅の実現可能性を実証する.
- RNA酵素を用いて逆向きのRNA分子を増幅する.
- 増幅RNA機能に基づくRNAポリメラーゼの進化の可能性を調査する.
主な方法:
- ポリメラーゼ連鎖反応 (PCR) に類似した熱循環を用いた.
- 機能性RNAと補完性RNAの両方の構成要素を供給した.
- 大量溶液と油中の水溶液のドロップで放大した.
主要な成果:
- RNA分子のクロスキラル指数関数増幅を達成した.
- ハンマーヘッドリボ酵素のl-RNA形式をd-RNAポリメラーゼを用いて成功的に増幅した.
- 増幅されたl-ハマーヘッドリボ酵素がl-RNA基板に触媒的活性を持っていることが示された.
- 大量溶液と区画化されたエムルションシステムの両方で増幅を披露しました.
結論:
- 交叉キラル指数式増幅は,逆向きのRNA分子を生成する有効な方法である.
- この技術は,リボ酵素などの機能的なRNA分子の増幅を可能にします.
- 様々な環境でエナチオRNAを増幅する能力は,新しいRNAポリメラーゼの進化の道を開く.
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